从食物中毒暴发中分离出的产气荚膜梭菌菌株中质粒携带的肠毒素基因（cpe）的聚合酶链反应鉴定

PCR identification of the plasmid-borne enterotoxin gene (cpe) in Clostridium perfringens strains isolated from food poisoning outbreaks.

作者信息

Nakamura Masahiko, Kato Aya, Tanaka Daisuke, Gyobu Yotaku, Higaki Shuichi, Karasawa Tadahiro, Yamagishi Takayoshi

机构信息

Department of Laboratory Sciences, School of Health Sciences, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan.

出版信息

Int J Med Microbiol. 2004 Oct;294(4):261-5. doi: 10.1016/j.ijmm.2004.03.004.

DOI:10.1016/j.ijmm.2004.03.004

PMID:15532984

Abstract

It is generally accepted that Clostridium perfringens strains associated with food poisoning carry their enterotoxin gene, cpe, on the chromosome, while C. perfringens strains isolated from non-food-borne diseases, such as antibiotic-associated diarrhea and sporadic diarrhea, carry cpe on the plasmid. However, we recently encountered a food poisoning outbreak caused by C. perfringens bearing a plasmid cpe. We therefore investigated a total of 31 clinical and non-clinical C. perfringens strains to locate the cpe gene by PCR. The cpe of nine heat-sensitive (100 degrees C for 10min) strains isolated from three outbreaks of food poisoning were located on the plasmid, while those of six heat-resistant strains from other food poisoning outbreaks were located on the chromosome. Moreover, the cpe of 5 heat-sensitive strains isolated from healthy human feces and those of 11 heat-sensitive soil strains were also located on the plasmid. These findings indicate that heat-sensitive, cpe-plasmid-borne C. perfringens strains should not be disregarded as causative agents of food poisoning.

摘要

人们普遍认为，与食物中毒相关的产气荚膜梭菌菌株的肠毒素基因cpe位于染色体上，而从非食源性疾病（如抗生素相关性腹泻和散发性腹泻）中分离出的产气荚膜梭菌菌株的cpe位于质粒上。然而，我们最近遇到了一起由携带质粒cpe的产气荚膜梭菌引起的食物中毒暴发。因此，我们总共调查了31株临床和非临床产气荚膜梭菌菌株，通过聚合酶链反应（PCR）来定位cpe基因。从三次食物中毒暴发中分离出的9株热敏性（100℃处理10分钟）菌株的cpe位于质粒上，而从其他食物中毒暴发中分离出的6株耐热性菌株的cpe位于染色体上。此外，从健康人粪便中分离出的5株热敏性菌株以及11株热敏性土壤菌株的cpe也位于质粒上。这些发现表明，携带cpe质粒的热敏性产气荚膜梭菌菌株作为食物中毒的病原体不应被忽视。