脲酶在两种不同地衣共生藻细胞壁中的细胞化学定位。

Cytochemical location of urease in the cell wall of two different lichen phycobionts.

作者信息

Millanes A M, Fontaniella B, García M L, Solas M T, Vicente C, Legaz M E

机构信息

Laboratory of Plant Biology, Faculty of Biology, Complutense University, C/José Antonio Novias s/n, 28040 Madrid, Spain.

出版信息

Tissue Cell. 2004 Dec;36(6):373-7. doi: 10.1016/j.tice.2004.06.007.

DOI:10.1016/j.tice.2004.06.007

PMID:15533452

Abstract

The enzyme urease has been located in the cell wall of recently isolated phycobionts from Evernia prunastri and Xanthoria parietina lichens. Cytochemical detection is achieved by producing a black, electron-dense precipitate of cobalt sulfide proceeding from CO(2) evolved from urea in the presence of cobalt chloride. Cellular fractionation reveals that about 80% of total urease activity was associated to the cell wall on both phycobionts whereas only 20% was recovered as soluble protein.

摘要

脲酶已定位在最近从扁枝衣和橙黄网衣地衣中分离出的藻菌层细胞壁中。细胞化学检测是通过在氯化钴存在的情况下，由尿素分解产生的二氧化碳生成黑色、电子致密的硫化钴沉淀来实现的。细胞分级分离显示，两种藻菌层中约80%的总脲酶活性与细胞壁相关，而只有20%以可溶性蛋白形式回收。