尿样中甲基黄嘌呤的分离、预浓缩及高效液相色谱分析。

Separation, pre-concentration, and HPLC analysis of methylxanthines in urine samples.

作者信息

Zydroń Mirosława, Baranowski Jacek, Baranowska Irena

机构信息

Department of Analytical and General Chemistry, Silesian University of Technology, 44-100 Gliwice, Poland.

出版信息

J Sep Sci. 2004 Oct;27(14):1166-72. doi: 10.1002/jssc.200401841.

DOI:10.1002/jssc.200401841

PMID:15537072

Abstract

An SPE method, using RP18 phases, for the simultaneous extraction of caffeine, theobromine, theophylline, paraxanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, 1-methyluric acid, 1,3-dimethyluric acid, 1,7-dimethyluric acid, and 1,3,7-trimethyluric acid from urine has been developed. Besides a gradient HPLC system for the analysis of the compounds of interest on a LiChrosorb RP-18 (7 microm) column with mobile phase containing 0.05% aq. solution of trifluoroacetic acid and acetonitrile has been elaborated. The procedure has been successfully applied to the analysis of methylxanthines and methyluric acids in urine of patients with chronic asthma treated with theophylline and in urine of healthy subjects.

摘要

已开发出一种采用RP18相的固相萃取（SPE）方法，用于从尿液中同时提取咖啡因、可可碱、茶碱、副黄嘌呤、1-甲基黄嘌呤、3-甲基黄嘌呤、7-甲基黄嘌呤、1-甲基尿酸、1,3-二甲基尿酸、1,7-二甲基尿酸和1,3,7-三甲基尿酸。此外，还精心设计了一种梯度高效液相色谱系统，用于在LiChrosorb RP-18（7微米）柱上分析目标化合物，流动相含有0.05%的三氟乙酸水溶液和乙腈。该方法已成功应用于茶碱治疗的慢性哮喘患者尿液以及健康受试者尿液中甲基黄嘌呤和甲基尿酸的分析。