链脲佐菌素诱导的糖尿病大鼠脊髓中缓激肽B1受体的表达

Expression of kinin B1 receptors in the spinal cord of streptozotocin-diabetic rat.

作者信息

Ongali Brice, Campos Maria Martha, Petcu Mihai, Rodi Donata, Cloutier Frank, Chabot Jean-Guy, Thibault Gaétan, Couture Réjean

机构信息

Department of Physiology, Université de Montréal, Pavillon Paul-G.-Desmarais, 2960, Chemin de la Tour, Montréal, Québec, H3T 1J4, Canada.

出版信息

Neuroreport. 2004 Nov 15;15(16):2463-6. doi: 10.1097/00001756-200411150-00006.

DOI:10.1097/00001756-200411150-00006

PMID:15538175

Abstract

Previous studies have reported cardiovascular and nociceptive responses after intrathecal injection of kinin B1 receptor (B1R) agonists in the model of streptozotocin (STZ)-diabetic rat (diabetic). The aim of this study was to measure the early up-regulation of B1R binding sites and mRNA in the thoracic spinal cord of diabetic and control rats. Data show significant increases of specific B1R binding sites in the dorsal horn of diabetic rats 2 days (+315%), 7 days (+303%) and 21 days (+181%) after STZ treatment. Levels of mRNA were significantly increased (+68%) at 2 and 7 days but not at 21 days. These data bring the first molecular evidence for an early up-regulation of B1R in the spinal cord of diabetic rat.

摘要

先前的研究报道了在链脲佐菌素（STZ）诱导的糖尿病大鼠模型中鞘内注射缓激肽B1受体（B1R）激动剂后的心血管和伤害性反应。本研究的目的是测量糖尿病大鼠和对照大鼠胸段脊髓中B1R结合位点和mRNA的早期上调情况。数据显示，在STZ治疗后2天（+315%）、7天（+303%）和21天（+181%），糖尿病大鼠背角中特异性B1R结合位点显著增加。mRNA水平在2天和7天时显著升高（+68%），但在21天时没有升高。这些数据为糖尿病大鼠脊髓中B1R的早期上调提供了首个分子证据。