使用庚醛改性壳聚糖从油桐叶中分离黄酮类化合物。
Separation of flavonoids from Aleurites moluccana leaves using chitosan modified with heptaldehyde.
作者信息
Morsch Michele, Girardi Leury G J, Cechinel-Filho Valdir, Meyre-Silva Christiane, Rodrigues Clóvis Antonio
机构信息
Núcleo de Investigações Químico-Farmacêuticas, Curso de Farmácia/CCS, Universidade do Vale do Itajaí, CEP 88.302-202, Itajaí, SC, Brasil.
出版信息
Z Naturforsch C J Biosci. 2004 Sep-Oct;59(9-10):649-52. doi: 10.1515/znc-2004-9-1007.
Heptaldehyde-modified chitosan (heptyl-chitosan, CH-Hp) was investigated as adsorbent for chromatograhic separation of the flavonoids from A. moluccana. The amount of 2"-O-rhamnosylswertisin isolated (30.0 mg) was approx. twice as high as swertisin (17.5 mg). The improved surface hydrophobicity effected by the heptyl groups promoted the separation of flavonoids. From the results obtained, CH-Hp seems to be more suitable for separation of glycosylated flavonoids than other flavonoids. Thus, modified chitosan described here can be used for hydrophobic interaction chromatography as sucessfully illustrated with flavonoids.
研究了庚醛修饰的壳聚糖（庚基壳聚糖，CH-Hp）作为从毛叶山柑中分离黄酮类化合物的吸附剂。分离得到的2″-O-鼠李糖基獐牙菜苷的量（30.0毫克）约为獐牙菜苷（17.5毫克）的两倍。庚基基团引起的表面疏水性增强促进了黄酮类化合物的分离。从所得结果来看，CH-Hp似乎比其他黄酮类化合物更适合分离糖基化黄酮类化合物。因此，本文所述的改性壳聚糖可成功用于疏水相互作用色谱法，黄酮类化合物就是很好的例证。
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