多个由组织特异性核因子识别的POU结合基序对神经干细胞中Dll1基因的表达很重要。

Multiple POU-binding motifs, recognized by tissue-specific nuclear factors, are important for Dll1 gene expression in neural stem cells.

作者信息

Nakayama Kohzo, Nagase Kazuko, Tokutake Yuriko, Koh Chang-Sung, Hiratochi Masahiro, Ohkawara Takeshi, Nakayama Noriko

机构信息

Department of Anatomy, Shinshu University, School of Medicine, Matsumoto, Nagano 390-8621, Japan.

出版信息

Biochem Biophys Res Commun. 2004 Dec 17;325(3):991-6. doi: 10.1016/j.bbrc.2004.10.138.

DOI:10.1016/j.bbrc.2004.10.138

PMID:15541387

Abstract

We cloned the 5'-flanking region of the mouse homolog of the Delta gene (Dll1) and demonstrated that the sequence between nucleotide position -514 and -484 in the 5'-flanking region of Dll1 played a critical role in the regulation of its tissue-specific expression in neural stem cells (NSCs). Further, we showed that multiple POU-binding motifs, located within this short sequence of 30bp, were essential for transcriptional activation of Dll1 and also that multiple tissue-specific nuclear factors recognized these POU-binding motifs in various combinations through differentiation of NSCs. Thus, POU-binding factors may play an important role in Dll1 expression in developing NSCs.

摘要

我们克隆了Delta基因（Dll1）小鼠同源物的5'侧翼区域，并证明Dll1的5'侧翼区域中核苷酸位置-514至-484之间的序列在神经干细胞（NSC）中其组织特异性表达的调控中起关键作用。此外，我们表明位于这一30bp短序列内的多个POU结合基序对于Dll1的转录激活至关重要，并且多个组织特异性核因子在NSC分化过程中通过不同组合识别这些POU结合基序。因此，POU结合因子可能在发育中的NSC的Dll1表达中起重要作用。