Sáez-Nieto J A, Lujan R, Berrón S, Campos J, Viñas M, Fusté C, Vazquez J A, Zhang Q Y, Bowler L D, Martinez-Suarez J V
Laboratorio de Referencia de Meningococos, Centro Nacional de Microbiología, Madrid, Spain.
Clin Infect Dis. 1992 Feb;14(2):394-402. doi: 10.1093/clinids/14.2.394.
Penicillin-resistant (penr) clinical isolates of Neisseria meningitidis, which do not produce beta-lactamase, were first identified in Spain in 1985; the frequency of their recovery, which has been increasing in the past few years, reached 20% in 1989. Serogrouping, determination of serotypes and subtypes, and multilocus enzyme electrophoresis of the penr strains showed an extensive diversity. Resistance is due, at least in part, to a decreased affinity of penicillin-binding protein (PBP) 2 for penicillin. Similar low-affinity forms of PBP 2 are also found in penr isolates of Neisseria lactamica, Neisseria polysaccharea, and Neisseria gonorrhoeae. Genetic transformation of an N. meningitidis type strain to low-level penicillin resistance with DNA from resistant meningococci and other Neisseria species resulted in transformants that possessed low-affinity forms of PBP 2. These altered forms of PBP 2 have been shown to arise from recombinational events that replace parts of the PBP 2 gene with the corresponding regions from the PBP 2 genes of commensal Neisseria species.
1985年在西班牙首次发现了不产生β-内酰胺酶的耐青霉素（penr）脑膜炎奈瑟菌临床分离株；在过去几年中其检出频率不断增加，1989年达到了20%。对penr菌株进行血清群分型、血清型和亚型测定以及多位点酶电泳分析，结果显示其具有广泛的多样性。耐药性至少部分是由于青霉素结合蛋白（PBP）2对青霉素的亲和力降低所致。在乳酸奈瑟菌、多糖奈瑟菌和淋病奈瑟菌的penr分离株中也发现了类似的低亲和力形式的PBP 2。用来自耐药脑膜炎球菌和其他奈瑟菌属物种的DNA将一株脑膜炎奈瑟菌标准菌株遗传转化为低水平青霉素耐药性，得到的转化体具有低亲和力形式的PBP 2。这些改变形式的PBP 2已被证明是由重组事件产生的，这些重组事件用共生奈瑟菌属物种PBP 2基因的相应区域取代了PBP 2基因的部分区域。
