[可溶性程序性死亡受体1可增强荷瘤小鼠中热休克蛋白70-肽复合物诱导的抗肿瘤免疫]
[Soluble PD-1 can augment anti-tumor immunity induced by HSP70-peptide complex in tumor-bearing mice].
作者信息
Wang Xiao-hong, Zhang Gui-mei, He Yu-fei, Zhang Hui, Feng Zuo-hua
机构信息
Department of Biochemistry & Molecular Biology, Tongji Medical College of Huazhong University of Sciences and Technology, Wuhan 430030, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Nov;20(6):655-8.
AIM
To explore the effect of soluble PD-1 (sPD-1) on the anti-tumor immunity induced by HSP70-peptide.
METHODS
BALB/c mice were immunized with HSP70-peptide, then the cytotoxic activity of splenocytes was detected by MTT colorimetry. Expression of PD-1 and its ligand on splenocytes was determined by semi-quantitative RT-PCR. For some mice, sPD-1 plasmid was injected alone or in combination with HSP70-peptide and then the tumor weight and cytotoxic activity of splenocytes were measured.
RESULTS
sPD-1 alone had anti-tumor effect. HSP70-peptide complex not only induced tumor-specific cytotoxic T lymphocytes(CTLs), but also promoted expression of PD-1 and its ligand. The combination of sPD-1 with HSP70-peptide complex induced much more stronger anti-tumor effect as compared with sPD-1 or HSP70-peptide alone. sPD-1 transfection in mice could enhance the cytotoxic activity of splenocytes from immunized mice.
CONCLUSION
sPD-1 enhanced the anti-tumor efficacy of HSP70-peptide.
目的
探讨可溶性程序性死亡蛋白1（sPD-1）对热休克蛋白70（HSP70）-肽诱导的抗肿瘤免疫的影响。
方法
用HSP70-肽免疫BALB/c小鼠，然后通过MTT比色法检测脾细胞的细胞毒活性。采用半定量逆转录聚合酶链反应（RT-PCR）测定脾细胞上PD-1及其配体的表达。对部分小鼠单独注射sPD-1质粒或与HSP70-肽联合注射，然后测量肿瘤重量和脾细胞的细胞毒活性。
结果
单独使用sPD-1具有抗肿瘤作用。HSP70-肽复合物不仅诱导肿瘤特异性细胞毒性T淋巴细胞（CTL），还促进PD-1及其配体的表达。与单独使用sPD-1或HSP70-肽相比，sPD-1与HSP70-肽复合物联合诱导出更强的抗肿瘤作用。小鼠体内sPD-1转染可增强免疫小鼠脾细胞的细胞毒活性。
结论
sPD-1增强了HSP70-肽的抗肿瘤疗效。
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