来自西非几内亚比绍的鸟分枝杆菌复合群临床分离株的遗传多样性。

Genetic diversity in clinical isolates of Mycobacterium avium complex from Guinea-Bissau, West Africa.

作者信息

Koivula Tuija, Cristea-Fernström Mariana, Chryssanthou Erja, Petrini Björn, Källenius Gunilla

机构信息

Department of Bacteriology, Swedish Institute for Infectious Disease Control, Nobels väg 18, SE-17182 Solna, Sweden.

出版信息

Microbes Infect. 2004 Nov;6(14):1320-5. doi: 10.1016/j.micinf.2004.07.007.

DOI:10.1016/j.micinf.2004.07.007

PMID:15555539

Abstract

Isolates of Mycobacterium avium complex (MAC) were cultured from sputum samples obtained from patients in Guinea-Bissau, West Africa. Twenty-eight isolates hybridising with MAC probe (AccuProbe) were further characterised by different molecular techniques: hybridisation with species-specific probes (AccuProbe) for M. avium and M. intracellulare, partial sequencing of 16S rRNA gene and PCR detection of the DT1-DT6 sequences and the macrophage-induced gene (mig). Only one of the 28 isolates reacted with the M. avium probe and four with the M. intracellulare probe. Two isolates expressed the DT1 sequence, and three the DT6. The mig was detected in 18 (64%) of the isolates. Sequencing of 16S rRNA had the greatest discriminative power of the typing methods applied, without strong correlation with any other technique. Clinical MAC isolates from Guinea-Bissau demonstrated a wide genetic diversity among the members of M. avium complex that might reflect on biotope variation.

摘要

从西非几内亚比绍患者的痰液样本中培养出鸟分枝杆菌复合群（MAC）菌株。通过不同的分子技术对28株与MAC探针（AccuProbe）杂交的菌株进行了进一步鉴定：与鸟分枝杆菌和胞内分枝杆菌的种特异性探针（AccuProbe）杂交、16S rRNA基因的部分测序以及DT1 - DT6序列和巨噬细胞诱导基因（mig）的PCR检测。28株菌株中只有1株与鸟分枝杆菌探针反应，4株与胞内分枝杆菌探针反应。2株菌株表达DT1序列，3株表达DT6。18株（64%）菌株中检测到mig。在应用的分型方法中，16S rRNA测序具有最大的鉴别能力，与任何其他技术均无强相关性。来自几内亚比绍的临床MAC分离株在鸟分枝杆菌复合群成员中表现出广泛的遗传多样性，这可能反映了生物群落的差异。