Soto M, Requena J M, Gomez L C, Navarrete I, Alonso C
Centro de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Eur J Biochem. 1992 Apr 1;205(1):211-6. doi: 10.1111/j.1432-1033.1992.tb16770.x.
A Leishmania donovani infantum promastigote cDNA expression library was screened with a serum obtained from a dog naturally infected with this parasite. One of the positive clones obtained revealed nucleotide sequence similarities with the histone H2A genes from various organisms. Northern blot analyses and sequence data of three independently isolated cDNA clones indicated that the Leishmania H2A mRNAs are polyadenylated, as are the basal histone mRNAs of higher eukaryotes and the histone mRNAs of yeast. The analysis of the genomic distribution of the DNA coding for histone H2A suggested that, in L. d. infantum, there are at least four genes coding for the H2A protein. It is likely that there is a simultaneous expression of at least two of the H2A genes since differences in nucleotide sequence between two of the sequenced cDNAs were observed. Affinity-purified antibodies against the beta-galactosidase-fused H2A protein recognize specifically a Leishmania protein band with a molecular mass of 14 kDa.
用从自然感染杜氏利什曼原虫婴儿亚种的犬血清筛选杜氏利什曼原虫婴儿亚种前鞭毛体cDNA表达文库。获得的一个阳性克隆显示与来自各种生物体的组蛋白H2A基因有核苷酸序列相似性。对三个独立分离的cDNA克隆的Northern印迹分析和序列数据表明，利什曼原虫H2A mRNA是多聚腺苷酸化的，高等真核生物的基础组蛋白mRNA和酵母的组蛋白mRNA也是如此。对编码组蛋白H2A的DNA的基因组分布分析表明，在婴儿杜氏利什曼原虫中，至少有四个基因编码H2A蛋白。由于观察到两个测序cDNA之间的核苷酸序列差异，很可能至少有两个H2A基因同时表达。针对β-半乳糖苷酶融合H2A蛋白的亲和纯化抗体特异性识别一条分子量为14 kDa的利什曼原虫蛋白条带。
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