从精浆中纯化具有酶活性的前列腺特异性抗原的两步法。

Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma.

作者信息

Bindukumar B, Kawinski Elzbieta, Cherrin Craig, Gambino Leah M, Nair Madhavan P N, Schwartz Stanley A, Chadha Kailash C

机构信息

Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 25;813(1-2):113-20. doi: 10.1016/j.jchromb.2004.09.014.

DOI:10.1016/j.jchromb.2004.09.014

PMID:15556523

Abstract

The role of prostate-specific antigen (PSA) during the onset of prostate cancer and subsequent tumor growth and metastasis is not well understood. We have developed a simple two step procedure, based on principles of hydrophobic charge-induction chromatography and molecular size chromatography to provide pure free-PSA (f-PSA) preparation that is free from all other known PSA complexes as well as human kallikrein 2 (hK2). The overall recovery of f-PSA is 72%. The isolated f-PSA consists of three known isoforms that corresponds to pI of 6.2, 6.4 and 7.2. f-PSA is enzymatically active and its enzymatic activity can be effectively neutralized by a serine protease inhibitor.

摘要

前列腺特异性抗原（PSA）在前列腺癌发生以及随后的肿瘤生长和转移过程中的作用尚未完全明确。我们基于疏水电荷诱导色谱法和分子大小色谱法的原理，开发了一种简单的两步法，以制备纯净的游离PSA（f-PSA），该f-PSA不含所有其他已知的PSA复合物以及人激肽释放酶2（hK2）。f-PSA的总回收率为72%。分离出的f-PSA由三种已知的异构体组成，其对应的等电点分别为6.2、6.4和7.2。f-PSA具有酶活性，其酶活性可被丝氨酸蛋白酶抑制剂有效中和。