Yoon Kyung-Hwan, Lee So-Young, Kim Won, Park Jong-Sei, Kim Hie-Joon
LabFrontier, KSBC Bldg, Iui-dong, Youngtong-ku, Kyunggi-do 442-270, South Korea.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 25;813(1-2):121-7. doi: 10.1016/j.jchromb.2004.09.018.
A simple, fast and sensitive high-performance liquid chromatography (HPLC)-mass spectrometric (MS) method has been developed for simultaneous determination of amoxicillin and clavulanic acid in human plasma using terbutaline as internal standard. After precipitation of the plasma proteins with acetonitrile, the analytes were separated on a C(8) reversed-phase column with formic acid-water-acetonirile (2:1000:100) and detected using electrospray ionization (ESI) mass spectrometry in negative selected ion monitoring (SIM) mode. The method was validated and successfully applied to analysis of amoxicillin and clavulanic acid in clinical studies. The limit of quantitation, 0.12 microg/ml for amoxicillin and 0.062 microg/ml for clavulanic acid, was five times lower than that of the published HPLC-UV method.
已开发出一种简单、快速且灵敏的高效液相色谱（HPLC）-质谱（MS）方法，以特布他林作为内标物，同时测定人血浆中的阿莫西林和克拉维酸。用乙腈沉淀血浆蛋白后，分析物在C(8)反相柱上以甲酸-水-乙腈（2:1000:100）进行分离，并采用电喷雾电离（ESI）质谱在负选择离子监测（SIM）模式下进行检测。该方法经过验证，并成功应用于临床研究中阿莫西林和克拉维酸的分析。阿莫西林的定量限为0.12微克/毫升，克拉维酸的定量限为0.062微克/毫升，比已发表的HPLC-UV方法低五倍。
Zotero 插件