含有2'-脱氧肌苷和2'-脱氧黄苷的三链螺旋DNA的热稳定性

Thermal stability of triple helical DNAs containing 2'-deoxyinosine and 2'-deoxyxanthosine.

作者信息

Ueno Yoshihito, Shibata Aya, Matsuda Akira, Kitade Yukio

机构信息

Department of Biomolecular Science, Faculty of Engineering, Gifu University, Laboratory of Molecular Biochemistry, Yanagido 501-1193, Gifu, Japan.

出版信息

Bioorg Med Chem. 2004 Dec 15;12(24):6581-6. doi: 10.1016/j.bmc.2004.09.024.

DOI:10.1016/j.bmc.2004.09.024

PMID:15556774

Abstract

In this paper, we describe the synthesis and thermal stabilities of the triplexes containing either 2'-deoxyinosine (1) or 2'-deoxyxanthosine (3) in their second strands. It was found that the triplexes with the 2'-deoxy-5-methylcytidine(dM)1:dC and dM1:dA base triplets are thermally stable, but those containing the dM1:T and dM1:dG base triplets are unstable under both neutral and slightly acidic conditions. On the other hand, it was found that the oligonucleotide containing 3 could form thermally stable triplexes with the oligonucleotides that involve four natural bases opposite the sites of 3. The rank of the thermal stabilities of the triplexes was as follows: the triplex containing the dM3:dC base triplet > that containing the dM3:dA base triplet > that containing the dM3:T base triplet > that containing the dM3:dG base triplet.

摘要

在本文中，我们描述了在第二条链中含有2'-脱氧肌苷（1）或2'-脱氧黄苷（3）的三链体的合成及其热稳定性。结果发现，具有2'-脱氧-5-甲基胞苷（dM）1:dC和dM1:dA碱基三联体的三链体具有热稳定性，但含有dM1:T和dM1:dG碱基三联体的三链体在中性和微酸性条件下均不稳定。另一方面，发现含有3的寡核苷酸可与在3位点相对处涉及四个天然碱基的寡核苷酸形成热稳定的三链体。三链体热稳定性的顺序如下：含有dM3:dC碱基三联体的三链体>含有dM3:dA碱基三联体的三链体>含有dM3:T碱基三联体的三链体>含有dM3:dG碱基三联体的三链体。