Chaabane L, Pellet N, Bourdillon M C, Desbleds Mansard C, Sulaiman A, Hadour G, Thivolet-Béjui F, Roy P, Briguet A, Douek P, Canet Soulas E
Laboratoire de RMN UMR CNRS 5012, UCB Lyon1-ESCPE, 69622, Villeurbanne, France.
MAGMA. 2004 Dec;17(3-6):188-95. doi: 10.1007/s10334-004-0055-7. Epub 2004 Nov 25.
To develop an MRI method for the evaluation of contrast enhancement in early atherosclerotic plaque development in the abdominal aorta of a mouse model. Male apoE-/- mice from three groups, respectively 4 (n = 6), 8 (n = 11) and 16 (n = 4) weeks were included. Axial T1 spin echo images of the abdominal aorta were obtained above and below the renal arteries (90 microm spatial resolution) before and over 1 h after the injection of a macromolecular contrast agent. Signal enhancement was measured in the vessel wall and compared to histological features. Maximal arterial wall signal enhancement was obtained from 16 to 32 min post injection. During this time, the signal-to-noise ratio increased by a factor up to 1.7 in 16 week mice and 2.7 and 2.4 in 8 and 4 weeks mice, respectively. The enhancement of the arterial wall appeared less pronounced in the oldest mice, 16 weeks old, exhibiting more advanced lesions. Using a macromolecular gadolinium agent, contrast uptake in atherogenesis varies with lesion stage and may be related to vessel-wall permeability. Dynamic contrast-enhanced MRI may be useful to evaluate the atherosclerotic plaque activity in mice.
开发一种磁共振成像（MRI）方法，用于评估小鼠模型腹主动脉早期动脉粥样硬化斑块形成过程中的对比增强情况。纳入来自三组的雄性载脂蛋白E基因敲除（apoE-/-）小鼠，分别为4周龄（n = 6）、8周龄（n = 11）和16周龄（n = 4）。在注射大分子对比剂之前以及注射后1小时内，获取肾动脉上方和下方腹主动脉的轴向T1自旋回波图像（空间分辨率为90微米）。测量血管壁中的信号增强情况，并与组织学特征进行比较。注射后16至32分钟获得最大动脉壁信号增强。在此期间，16周龄小鼠的信噪比提高了1.7倍，8周龄和4周龄小鼠的信噪比分别提高了2.7倍和2.4倍。在16周龄的最年长小鼠中，动脉壁的增强似乎不太明显，其病变更为严重。使用大分子钆剂时，动脉粥样硬化形成过程中的对比剂摄取随病变阶段而变化，可能与血管壁通透性有关。动态对比增强MRI可能有助于评估小鼠动脉粥样硬化斑块的活性。
