Zilberstein Gleb V, Baskin Emmanuil M, Bukshpan Shmuel, Korol Leonid E
Protein Forest Inc., Rehovot, Israel.
Electrophoresis. 2004 Nov;25(21-22):3643-51. doi: 10.1002/elps.200406117.
A miniature electrophoretic device is developed on the basis of a new isoelectric focusing (IEF) method, namely parallel isoelectric focusing. We report here the theory and the results of operation of a new parallel isoelectric device (PID). The main advantages and limitations of the method are discussed for miniaturization purposes. It is shown that the method guarantees the fast and complete separation of any complex protein mixtures under acceptable conditions, such as voltage source, temperature, size of the device, and separation process duration. It is shown that the main problem of PID miniaturization is the buffer design, and the relation between Immobiline buffer capacity and solution buffer capacity. The main experimental limitation of PID resolution is protein sensitivity to pH changes.
一种微型电泳装置是基于一种新的等电聚焦（IEF）方法——平行等电聚焦开发的。我们在此报告一种新型平行等电装置（PID）的理论及运行结果。为了小型化目的，讨论了该方法的主要优点和局限性。结果表明，该方法能在可接受的条件下（如电压源、温度、装置尺寸和分离过程持续时间）保证快速、完全地分离任何复杂蛋白质混合物。结果表明，PID小型化的主要问题是缓冲液设计以及固定化缓冲液容量与溶液缓冲液容量之间的关系。PID分辨率的主要实验限制是蛋白质对pH变化的敏感性。
