阿尔茨海默病患者死后颞叶皮质中hnRNP B1的免疫组织化学研究

Immunohistochemical study of hnRNP B1 in the postmortem temporal cortices of patients with Alzheimer's disease.

作者信息

Ishikawa Masanori, Mizukami Katsuyoshi, Iwakiri Masahiko, Kamma Hiroshi, Ikonomovic Milos D, Dekosky Steven T, Asada Takashi

机构信息

Department of Psychiatry, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba city, Ibaraki 305-8575, Japan.

出版信息

Neurosci Res. 2004 Dec;50(4):481-4. doi: 10.1016/j.neures.2004.08.013.

DOI:10.1016/j.neures.2004.08.013

PMID:15567486

Abstract

In order to examine the post-transcriptional regulations in Alzheimer's disease, we employed immunohistochemical techniques and examined the expression of heterogeneous nuclear ribonucleoprotein (hnRNP) B1 in the inferior temporal cortex of subjects with Alzheimer's disease. In the mild cases, intense B1 immunoreactivity was observed in neurons of layer V, and less intense immunoreactivity was observed in layers II and III. The overall distributions and intensities of B1 immunoreactivity were undistinguishable among mild, moderate, and severe cases. Double-immunolabeling with MC1 and B1 demonstrated that B1 immunoreactivity was preserved in the majority of neurofibrillary tangle (NFT)-bearing neurons. Our study suggests that hnRNP B1-associated post-transcriptional regulations are preserved in the inferior temporal cortex of Alzheimer's disease.

摘要

为了研究阿尔茨海默病中的转录后调控，我们采用免疫组织化学技术，检测了阿尔茨海默病患者颞下回皮质中异质性核糖核蛋白（hnRNP）B1的表达。在轻度病例中，在V层神经元中观察到强烈的B1免疫反应性，而在II层和III层中观察到较弱的免疫反应性。在轻度、中度和重度病例中，B1免疫反应性的总体分布和强度没有差异。用MC1和B1进行双重免疫标记表明，在大多数含有神经原纤维缠结（NFT）的神经元中，B1免疫反应性得以保留。我们的研究表明，hnRNP B1相关的转录后调控在阿尔茨海默病的颞下回皮质中得以保留。