Phadtare Sumant, Vyas Santosh, Palaskar Dnyaneshwar V, Lachke Anil, Shukla Parshuram G, Sivaram Swaminathan, Sastry Murali
Materials Chemistry, Biochemical Sciences, and Polymer Chemistry Divisions, National Chemical Laboratory, Pune 411 008, India.
Biotechnol Prog. 2004 Nov-Dec;20(6):1840-6. doi: 10.1021/bp0499000.
The synthesis of polyurethane microsphere-gold nanoparticle "core-shell" structures and their use in the immobilization of the enzyme endoglucanase are described. Assembly of gold nanoparticles on the surface of polymer microspheres occurs through interaction of the nitrogens in the polymer with the nanoparticles, thereby precluding the need for modifying the polymer microspheres to enable such nanoparticle binding. Endoglucanse could thereafter be bound to the gold nanoparticles decorating the polyurethane microspheres, leading to a highly stable biocatalyst with excellent reuse characteristics. The immobilized enzyme retains its biocatalytic activity and exhibits improved thermal stability relative to free enzyme in solution. The high surface area of the host gold nanoparticles renders the immobilized enzyme "quasi free", while at the same time retaining advantages of immobilization such as ease of reuse, enhanced temporal and thermal stability, etc.
本文描述了聚氨酯微球-金纳米粒子“核壳”结构的合成及其在固定化内切葡聚糖酶中的应用。金纳米粒子通过聚合物中的氮与纳米粒子的相互作用在聚合物微球表面组装，从而无需对聚合物微球进行改性以实现这种纳米粒子结合。此后，内切葡聚糖酶可以与装饰聚氨酯微球的金纳米粒子结合，从而得到具有优异重复使用特性的高度稳定的生物催化剂。相对于溶液中的游离酶，固定化酶保留了其生物催化活性并表现出更高的热稳定性。主体金纳米粒子的高表面积使固定化酶“准游离”，同时保留了固定化的优点，如易于重复使用、增强的时间和热稳定性等。
