一项关于BIV Tat肽与其TAR RNA双链体、RNA-DNA异源双链体及DNA双链体的比较结合研究。

A comparative binding study of BIV Tat peptide against its TAR RNA duplex, RNA-DNA heteroduplex and DNA duplex.

作者信息

Tok Jeffrey B-H, Bi Lanrong

机构信息

Department of Chemistry, York College and Graduate Center, The City University of New York (CUNY), 94-20 Guy R. Brewer Blvd., Jamaica, NY 11451, USA.

出版信息

Bioorg Med Chem Lett. 2005 Jan 3;15(1):129-33. doi: 10.1016/j.bmcl.2004.10.030.

DOI:10.1016/j.bmcl.2004.10.030

PMID:15582425

Abstract

Association between RNA and DNA strands to form RNA-DNA heteroduplex is important in many biological processes such as transcription, DNA replication and reverse transcription. Herein, binding affinities of a 17-mer BIV Tat peptide is compared with TAR DNA duplex, TAR RNA-DNA heteroduplex and TAR RNA duplex. It was observed that binding affinities of Tat peptide is comparable against DNA-RNA heteroduplex and RNA duplex, whereas DNA duplex binding is decidedly poor.

摘要

RNA与DNA链结合形成RNA-DNA异源双链体在许多生物学过程中都很重要，如转录、DNA复制和逆转录。在此，将17聚体BIV Tat肽与TAR DNA双链体、TAR RNA-DNA异源双链体和TAR RNA双链体的结合亲和力进行了比较。结果发现，Tat肽与DNA-RNA异源双链体和RNA双链体的结合亲和力相当，而与DNA双链体的结合则明显较差。