外源性人绒毛膜促性腺激素对培养的输卵管黏膜细胞中血管内皮生长因子及其受体mRNA表达的上调作用。

Upregulation of mRNA expression of vascular endothelial growth factor and its receptors by exogenous human chorionic gonadotropin in cultured oviduct mucosal cells.

作者信息

Lam Po Mui, Briton-Jones Christine, Cheung Che Kwok, Cheung Lai Ping, Haines Christopher

机构信息

Department of Obstetrics and Gynecology, The Prince of Wales Hospital, Shatin, Hong Kong, SAR, China.

出版信息

Fertil Steril. 2004 Dec;82(6):1708-10. doi: 10.1016/j.fertnstert.2004.06.043.

DOI:10.1016/j.fertnstert.2004.06.043

PMID:15589889

Abstract

The mucosal cells were isolated from the ampullary regions of 20 human oviducts and cultured with or without hCG in five different concentrations (1-100 ng/mL). As analyzed by the semiquantitative reverse-transcriptase polymerase chain reaction, hCG treatment significantly increased mRNA expression of vascular endothelial growth factor and its receptor flt-1 in the cultured mucosal cells in a dose-dependent manner but had no effect on the expression of another receptor, KDR.

摘要

从20条人输卵管壶腹部区域分离出黏膜细胞，在五种不同浓度（1 - 100 ng/mL）的hCG存在或不存在的情况下进行培养。通过半定量逆转录聚合酶链反应分析，hCG处理以剂量依赖方式显著增加了培养的黏膜细胞中血管内皮生长因子及其受体flt - 1的mRNA表达，但对另一种受体KDR的表达没有影响。