Wilson Joanna Y, McArthur Andrew G, Stegeman John J
Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.
Gen Comp Endocrinol. 2005 Jan 1;140(1):74-83. doi: 10.1016/j.ygcen.2004.10.004.
Aromatase (cytochrome P450 19, CYP19, P450arom) is the enzyme responsible for the production of estrogens, hormones critical for development and reproduction. Aromatase was sequenced from a white-sided dolphin (Lagenorhynchus acutus) ovary, transiently transfected into HEK 293 cells, and the expressed protein was characterized for aromatase activity in the presence of androstenedione and testosterone and after exposure to the aromatase inhibitor letrazole. The Kms for androstenedione and testosterone were 63.5 and 75 nM, respectively, values that are very similar to those reported for other mammalian aromatases. A Bayesian phylogenetic analysis of the vertebrate aromatases was performed on the amino acid sequences of aromatases from fish, amphibians, reptiles, birds, and mammals. Based on known species phylogeny, the cetacean aromatase showed an expected grouping with artiodactyls (cow, sheep, and goat). An analysis of functional divergence showed strong conservation of aromatase across the entire protein, which indicates that the observed sequence divergence is functionally neutral.
芳香化酶（细胞色素P450 19，CYP19，P450arom）是负责产生雌激素的酶，雌激素是对发育和繁殖至关重要的激素。从白侧海豚（白喙斑纹海豚）卵巢中对芳香化酶进行测序，将其瞬时转染到HEK 293细胞中，并在存在雄烯二酮和睾酮的情况下以及在暴露于芳香化酶抑制剂来曲唑后，对表达的蛋白质进行芳香化酶活性表征。雄烯二酮和睾酮的米氏常数分别为63.5和75 nM，这些值与其他哺乳动物芳香化酶报道的值非常相似。基于鱼类、两栖动物、爬行动物、鸟类和哺乳动物的芳香化酶氨基酸序列，对脊椎动物芳香化酶进行了贝叶斯系统发育分析。基于已知的物种系统发育，鲸类芳香化酶与偶蹄目动物（牛、绵羊和山羊）呈现出预期的分组。功能差异分析表明，整个蛋白质中芳香化酶具有很强的保守性，这表明观察到的序列差异在功能上是中性的。
Zotero 插件