Soh Shigehiro, Ishii Toshiharu, Sato Erina, Akishima Yuri, Ito Kinji, Baba Shiro
Department of Urology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
Prostate. 2005 Jun 1;63(4):330-5. doi: 10.1002/pros.20199.
The lymphatic endothelial hyaluronan receptor (LYVE-1) is a specific cell surface protein in lymphatic endothelium. The antiserum against human LYVE-1 was developed and was confirmed a powerful marker of lymphatic endothelium in human organs. With this novel marker we investigated the small network of intraprostate lymphatic vessels.
To identify intraprostatic lymphatic vessels, we performed an immunohistochemical staining method using LYVE-1 pAb and von Willebrand Factor (vWF), and assessed the difference in distribution of small lymphatic vessels between the components in the prostate.
The density of lymphatic vessel was significantly high around ejaculatory ducts and in the fibromascular area between the globular area of peripheral zone and transitional zone. Predominat lymphatic vessels distributed in the fibromascular area in the anterior and posterior prostate of extrastromal area.
Recognition of the distinctive features of the intraprostate lymphatic network, can help the investigation of lymphatic involvement in cancer of the prostate.
淋巴管内皮透明质酸受体(LYVE-1)是淋巴管内皮中的一种特异性细胞表面蛋白。已研制出针对人LYVE-1的抗血清,并证实其是人体器官中淋巴管内皮的一种有效标志物。利用这一新标志物,我们研究了前列腺内淋巴管的小网络。
为识别前列腺内的淋巴管,我们采用LYVE-1多克隆抗体和血管性血友病因子(vWF)进行免疫组织化学染色方法,并评估前列腺各成分中小淋巴管分布的差异。
射精管周围以及外周区球状区域与移行区之间的纤维血管区域内淋巴管密度显著较高。主要的淋巴管分布在前列腺基质外区域的前后部纤维血管区域。
认识前列腺内淋巴网络的独特特征,有助于研究前列腺癌中的淋巴受累情况。