用来自红平红球菌的重组腈水合酶和酰胺酶对氰醇进行酶促水解。
Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from Rhodococcus erythropolis.
作者信息
Reisinger Ch, Osprian I, Glieder A, Schoemaker H E, Griengl H, Schwab H
机构信息
Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz, 8010, Austria.
出版信息
Biotechnol Lett. 2004 Nov;26(21):1675-80. doi: 10.1007/s10529-004-3521-4.
Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli. Crude cell free extracts were used for the hydrolysis of different aromatic cyanohydrins. Nitrile hydratase expression was increased up to 5-fold by redesign of the expression cassette. The recombinant enzymes were successfully used for the conversion of several cyanohydrins to the corresponding alpha-hydroxy amides and acids while retaining enantiopurity.
来自红平红球菌CIMB11540的腈水合酶和酰胺酶均在大肠杆菌中进行了克隆和表达。使用粗制无细胞提取物对不同的芳香氰醇进行水解。通过重新设计表达盒，腈水合酶的表达提高了5倍。重组酶成功用于将几种氰醇转化为相应的α-羟基酰胺和酸，同时保持对映体纯度。
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