用于直接分析液相色谱馏分中二苯并芘异构体的激光激发时间分辨Shpol'skii光谱法。
Laser-excited time-resolved Shpol'skii spectroscopy for the direct analysis of dibenzopyrene isomers in liquid chromatography fractions.
作者信息
Yu Shenjiang, Campiglia Andres D
机构信息
Department of Chemistry, P. O. Box 25000, University of Central Florida, Orlando, Florida 32816-2366, USA.
出版信息
Appl Spectrosc. 2004 Dec;58(12):1385-93. doi: 10.1366/0003702042641416.
We present a unique method for the unambiguous determination of dibenzo[a,l]pyrene, dibenzo[a,e]pyrene, dibenzo[a,h]pyrene, dibenzo[a,i]pyrene, and dibenzo[e,l]pyrene in high-performance liquid chromatography (HPLC) fractions. Chemical analysis is performed via laser-excited time-resolved Shpol'skii spectroscopy with the aid of a cryogenic fiber-optic probe, pulsed tunable dye laser, spectrograph, and intensified charge-coupled device. Unambiguous identification is accomplished via wavelength time matrix formats, which give simultaneous access to spectral and lifetime information. Prior to spectroscopic analysis, HPLC fractions are pre-treated with liquid-liquid extraction or solid-liquid extraction at the tip of the fiber-optic probe. Solid-liquid extraction gives the best limits of detection, which vary from 40 pg mL(-1) (dibenzo[a,l]pyrene) to 0.2 ng mL(-1)(dibenzo[e,l]pyrene).
我们提出了一种独特的方法，用于在高效液相色谱（HPLC）馏分中明确测定二苯并[a,l]芘、二苯并[a,e]芘、二苯并[a,h]芘、二苯并[a,i]芘和二苯并[e,l]芘。借助低温光纤探头、脉冲可调谐染料激光器、光谱仪和增强型电荷耦合器件，通过激光激发的时间分辨肖洛斯基光谱法进行化学分析。通过波长时间矩阵格式实现明确鉴定，该格式可同时获取光谱和寿命信息。在光谱分析之前，HPLC馏分在光纤探头尖端进行液-液萃取或固-液萃取预处理。固-液萃取给出了最佳检测限，范围从40 pg mL⁻¹（二苯并[a,l]芘）到0.2 ng mL⁻¹（二苯并[e,l]芘）。
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