重复电惊厥休克对大鼠额叶皮质中Cdk2-pRB-E2F1细胞周期通路的激活作用。
Activation of Cdk2-pRB-E2F1 cell cycle pathway by repeated electroconvulsive shock in the rat frontal cortex.
作者信息
Kim Yeni, Seo Myoung-Suk, Kang Ung-Gu, Yoon Se Chang, Ahn Yong-Min, Kim Yong Sik, Juhnn Yong-Sung
机构信息
Department of Psychiatry and Behavioral Science and Institute for Institute of Human Behavioral Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea.
出版信息
Biol Psychiatry. 2005 Jan 1;57(1):107-9. doi: 10.1016/j.biopsych.2004.10.025.
BACKGROUND
Recent reports indicate that repeated electroconvulsive shock (ECS) induces cortical cell proliferation, suggesting the possibility that ECS may activate cell cycle progression in the rat brain cortex.
METHODS
Sprague-Dawley rats (150-200g) were divided into four treatment groups and then given sham treatment or ECS treatment for 1, 5, and 10 days, respectively. The activity of cyclin-dependent kinase 2 (Cdk2), phosphorylation, and total protein amount of cyclin D1, cyclin E, pocket retinoblastoma family of protein (pRB), and E2F1 were analyzed in the rat cerebral cortex.
RESULTS
The activity of Cdk2, the protein amount of pRB, Ser795 phosphorylation of pRB, and the protein amount of E2F1 were all increased compared with the sham-treated control subjects, and these increases were enhanced with the increasing number of ECS. In contrast, the protein amounts of Cdk2, cyclin D1, and cyclin E were not changed by repeated ECS.
CONCLUSIONS
The Cdk2-pRB-E2F1 cell cycle pathway is activated by repeated ECS in the rat frontal cortex.
背景
最近的报告表明，重复电惊厥休克（ECS）可诱导皮质细胞增殖，这提示ECS可能激活大鼠大脑皮质中的细胞周期进程。
方法
将体重150 - 200克的Sprague-Dawley大鼠分为四个治疗组，然后分别给予假手术治疗或ECS治疗1天、5天和10天。分析大鼠大脑皮质中细胞周期蛋白依赖性激酶2（Cdk2）的活性、细胞周期蛋白D1、细胞周期蛋白E、视网膜母细胞瘤蛋白家族（pRB）和E2F1的磷酸化水平及总蛋白量。
结果
与假手术对照组相比，Cdk2的活性、pRB的蛋白量、pRB的Ser795磷酸化水平以及E2F1的蛋白量均增加，且随着ECS次数的增加而增强。相比之下，重复ECS并未改变Cdk2、细胞周期蛋白D1和细胞周期蛋白E的蛋白量。
结论
在大鼠额叶皮质中，重复ECS可激活Cdk2-pRB-E2F1细胞周期通路。
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