Notch配体Delta-1可改变视黄酸（RA）诱导的嗜中性粒细胞分化为单核细胞，并减少RA诱导的NB4细胞凋亡。

The Notch ligand, Delta-1, alters retinoic acid (RA)-induced neutrophilic differentiation into monocytic and reduces RA-induced apoptosis in NB4 cells.

作者信息

Murata-Ohsawa Mai, Tohda Shuji, Kogoshi Hanae, Sakano Seiji, Nara Nobuo

机构信息

Department of Laboratory Medicine, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-Ku, Tokyo 113-8519, Japan.

出版信息

Leuk Res. 2005 Feb;29(2):197-203. doi: 10.1016/j.leukres.2004.05.020.

DOI:10.1016/j.leukres.2004.05.020

PMID:15607369

Abstract

Effects of Notch activation on retinoic acid (RA)-induced differentiation and apoptosis were investigated. NB4, an acute promyelocytic leukemia (APL) cell line, undergoes neutrophilic differentiation and apoptosis by RA. Notch activation induced by a recombinant Notch ligand, Delta-1, did not affect the growth by itself. Treatment with RA plus Delta-1 made part of NB4 cells monocyte-like shaped and reduced the apoptosis. Similar phenomenon was also observed in primary APL cells. RA treatment induced cleavage of caspase-8 and PARP in NB4. Delta-1 suppressed the RA-induced cleavage of them, which may be a possible mechanism through which Delta-1 suppressed the RA-induced apoptosis.

摘要

研究了Notch激活对维甲酸（RA）诱导的分化和凋亡的影响。NB4是一种急性早幼粒细胞白血病（APL）细胞系，可通过RA进行嗜中性分化和凋亡。重组Notch配体Delta-1诱导的Notch激活本身并不影响细胞生长。用RA加Delta-1处理使部分NB4细胞呈单核细胞样形态并减少了凋亡。在原发性APL细胞中也观察到类似现象。RA处理诱导NB4中caspase-8和PARP的裂解。Delta-1抑制了RA诱导的它们的裂解，这可能是Delta-1抑制RA诱导的凋亡的一种可能机制。