Kasai Yasuhiro, Hashimoto Shin-ichi, Yamada Tomoyuki, Sese Jun, Sugano Sumio, Matsushima Kouji, Morishita Shinichi
Department of Computational Biology, Graduate School of Frontier Science, The University of Tokyo, 5-1-5 Kashinoha, Kashiwa City, Chiba 277-8562, Japan.
Nucleic Acids Res. 2005 Jan 1;33(Database issue):D550-2. doi: 10.1093/nar/gki085.
To comprehensively identify transcription start sites and the frequencies of individual mRNAs in human cell libraries, a method of 5' end Serial Analysis of Gene Expression (SAGE) was developed recently, which makes it possible to collect a large amount of start site information, and subsequently, we have established a related database server called 5'SAGE. This database displays the observed frequencies of individual 5' end SAGE tags and previously unknown transcription start sites in the promoter regions, introns and intergenic regions of known genes. 5'SAGE will be useful for analyzing promoter regions and start site variation in different tissues, and is freely available at http://5sage.gi.k.u-tokyo.ac.jp/.
为了全面鉴定人类细胞文库中转录起始位点和单个mRNA的频率,最近开发了一种5'端基因表达序列分析(SAGE)方法,该方法使得收集大量起始位点信息成为可能,随后,我们建立了一个名为5'SAGE的相关数据库服务器。该数据库展示了已知基因的启动子区域、内含子和基因间区域中单个5'端SAGE标签的观察频率以及先前未知的转录起始位点。5'SAGE将有助于分析不同组织中的启动子区域和起始位点变异,可在http://5sage.gi.k.u-tokyo.ac.jp/免费获取。