[重型再生障碍性贫血患者骨髓中DC1亚群的变化]

He Guang-sheng, Shao Zong-hong, He Hong, Liu Hong, Fu Rong, Bai Jie, Shi Jun, Cao Yan-ran, Tu Mei-feng, Sun Juan, Jia Hai-rong

Institute of Hematology and Blood Disease Hospital, CAMS and PUMC, Tianjin 300020, China.

Zhonghua Xue Ye Xue Za Zhi. 2004 Nov;25(11):649-52.

OBJECTIVE

To measure the subsets of dendritic cells 1 (DC1) in the bone marrow of severe aplastic anemia (SAA) patients and evaluate the relationships between the CD11c+CD83+ cells and Th1 cells, CD3+CD8+ cells or hematopoietic function and explore the role of DC1 in the pathogenesis of SAA.

METHODS

By FACS, the quantities and ratios of CD11c+CD1a+ cells, CD11c+CD83+ cells, Th1 cells, and CD3+CD8+ cells in the bone marrow of SAA patients and normal controls were detected respectively. The relationships between CD3+CD8+ cells and reticulocyte absolute value (Ret) or neutrophil absolute value (ANC), between Th1 cells and CD3+CD8+ cells, Ret or ANC, between CD11c+CD83+ cells, and Th1 cells, CD3+CD8+ cells, Ret or ANC were evaluated.

RESULTS

In normal controls' bone marrow, the percentages of Th1 cells, CD11c+CD1a+ cells, CD11c+CD83+ cells and the ratio of CD11c+CD83+/CD11c+CD1a+ were (0.42 +/- 0.30)%, (0.38 +/- 0.29)%, (0.37 +/- 0.32)% and 1.07 +/- 0.10, respectively. In untreated SAA patients, they were (4.87 +/- 0.54)%, (1.73 +/- 0.24)%, (3.38 +/- 0.56)% and 2.21 +/- 0.32 respectively, which were higher than that in normal controls (P < 0.01). In recovering SAA patients, the percentages of Th1 cells, CD11c+CD1a+ cells and CD11c+CD83+ cells decreased significantly to (0.53 +/- 0.22)%, (0.61 +/- 0.23)%, (0.65 +/- 0.22)%, respectively (P < 0.01). The ratio of CD11c+CD83+/CD11c+ CD1a+ in recovering SAA patients decreased to 1.37 +/- 0.25, which was similar to that in normal controls (P > 0.05). The percentage of CD3+CD8+ cells in untreated SAA patients was (32.32 +/- 10.22)%, and in recovering SAA patients decreased to (13.67 +/- 5.24)% (P < 0.01). The percentage of CD3+CD8+ cells in SAA patients was negatively correlated with their Ret and ANC (P < 0.05), while their Th1 cell percentages were positively correlated with their CD3+CD8+ cells (P < 0.01), and negatively correlated with their Ret and ANC (P < 0.01). SAA patient's CD11c+CD83+ cell percentages were positively correlated with their Th1 cell and CD3+CD8 cells (P < 0.01, P < 0.05), but negatively with their Ret and ANC (P < 0.01).

CONCLUSION

Both immature DC1 and activated DC1 increased in the bone marrow of SAA patients, and the balance of DC1 subsets shifted from stable form to active one, which might promote Th0 cells to polarize to Th1 cells, and cause the over-function of T lymphocytes and hematopoiesis failure in SAA.

目的

检测重型再生障碍性贫血（SAA）患者骨髓中树突状细胞1（DC1）亚群，评估CD11c+CD83+细胞与Th1细胞、CD3+CD8+细胞之间的关系以及与造血功能的关系，探讨DC1在SAA发病机制中的作用。

方法

采用流式细胞术分别检测SAA患者及正常对照骨髓中CD11c+CD1a+细胞、CD11c+CD83+细胞、Th1细胞及CD3+CD8+细胞的数量和比例。评估CD3+CD8+细胞与网织红细胞绝对值（Ret）或中性粒细胞绝对值（ANC）之间、Th1细胞与CD3+CD8+细胞、Ret或ANC之间、CD11c+CD83+细胞与Th1细胞、CD3+CD8+细胞、Ret或ANC之间的关系。

结果

正常对照骨髓中，Th1细胞、CD11c+CD1a+细胞、CD11c+CD83+细胞百分比及CD11c+CD83+/CD11c+CD1a+比值分别为（0.42±0.30）%、（0.38±0.29）%、（0.37±0.32）%和1.07±0.10。未治疗的SAA患者分别为（4.87±0.54）%、（1.73±0.24）%、（3.38±0.56）%和2.21±0.32，均高于正常对照（P<0.01）。病情恢复的SAA患者，Th1细胞、CD11c+CD1a+细胞及CD11c+CD83+细胞百分比显著下降至（0.53±0.22）%、（0.61±0.23）%、（0.65±0.22）%（P<0.01）。病情恢复的SAA患者CD11c+CD83+/CD11c+CD1a+比值降至1.37±0.25，与正常对照相似（P>0.05）。未治疗的SAA患者CD3+CD8+细胞百分比为（32.32±10.22）%，病情恢复的SAA患者降至（13.67±5.24）%（P<0.01）。SAA患者CD3+CD8+细胞百分比与其Ret和ANC呈负相关（P<0.05），而Th1细胞百分比与其CD3+CD8+细胞呈正相关（P<0.01），与Ret和ANC呈负相关（P<0.01）。SAA患者CD11c+CD83+细胞百分比与其Th1细胞及CD3+CD8细胞呈正相关（P<0.01，P<0.05），与Ret和ANC呈负相关（P<0.01）。