[生存素在结直肠癌中的表达及其与细胞凋亡和血管生成的关系的临床意义]

[Clinical significance of survivin expression in colorectal cancer and its relationship with cell apoptosis and angiogenesis].

作者信息

Yang Jing, Liu Feng-Xuan, Yan Xiao-Chu, He Guang-You, Liu Li-Mei

机构信息

Pathology Institute, Southwest Hospital, The Third Military Medical University, Chongqing 400-038, P.R. China.

出版信息

Ai Zheng. 2005 Jan;24(1):116-20.

PMID:15642214

Abstract

BACKGROUND & OBJECTIVE: Expression of survivin, an apoptosis suppressor gene, in colorectal cancer(CRC), and its relationship with pathologic factors, cell apoptosis, and angiogenesis are unclear. This study aimed to investigate the effect of survivin on cell apoptosis, and its relation with angiogenesis, and to further explore the effects of survivin on development and prognosis of CRC.

METHODS

Protein expression of survivin, and vascular endothelial growth factor (VEGF) in 91 specimens of CRC was detected by immunohistochemistry, apoptosis index (AI) of tumor cells was detected by TUNEL method.

RESULTS

Positive rate of survivin in CRC with distant metastasis was 56.0% (14/25), significantly higher than that in CRC without metastasis (48.5%, 32/66) (P<0.05); and that in 5-year survival patients(42.9%,30/70) was lower than that in patients died within 5 years (76.2%,16/21) (P<0.01). The average survival time of patients with positive expression of survivin was 91.3 months, and that of patients with negative expression of survivin was 116.4 months; 5-year survival rate of the former (65.2%, 30/46) was significantly lower than that of the latter (88.9%, 40/45) (P<0.01). The average AI of patients with positive expression of survivin was lower than that of patients with negative expression of survivin [(0.74+/-0.19)% vs. (1.07+/-0.24)%, P<0.01]; the expression of survivin significantly correlated with that of VEGF (pearson: 0.721).

CONCLUSION

Survivin may promote metastasis, and affect prognosis of CRC through inhibiting cell apoptosis, and regulating angiogenesis of CRC.

摘要

背景与目的

凋亡抑制基因survivin在结直肠癌（CRC）中的表达及其与病理因素、细胞凋亡和血管生成的关系尚不清楚。本研究旨在探讨survivin对细胞凋亡的影响及其与血管生成的关系，并进一步探讨survivin对CRC发生发展及预后的影响。

方法

采用免疫组织化学法检测91例CRC标本中survivin和血管内皮生长因子（VEGF）的蛋白表达，采用TUNEL法检测肿瘤细胞凋亡指数（AI）。

结果

远处转移的CRC中survivin阳性率为56.0%（14/25），显著高于无转移的CRC（48.5%，32/66）（P<0.05）；5年生存患者中的阳性率（42.9%，30/70）低于5年内死亡患者（76.2%，16/21）（P<0.01）。survivin阳性表达患者的平均生存时间为91.3个月，survivin阴性表达患者的平均生存时间为116.4个月；前者的5年生存率（65.2%，30/46）显著低于后者（88.9%，40/45）（P<0.01）。survivin阳性表达患者的平均AI低于survivin阴性表达患者[（0.74±0.19）%对（1.07±0.24）%，P<0.01]；survivin的表达与VEGF的表达显著相关（pearson相关系数：0.721）。