通过原子力显微镜（AFM）和落射荧光显微镜研究了有机磷水解酶（OPH）与对氧磷在气-水界面的相互作用。

The interaction between OPH and paraoxon at the air-water interface studied by AFM and epifluorescence microscopies.

作者信息

Cao Xihui, Mabrouki Mustapha, Mello Sarita V, Leblanc Roger M, Rastogi Vipin K, Cheng Tu-Chen, DeFrank Joseph J

机构信息

Department of Chemistry, University of Miami, Coral Gables, FL 33124, USA.

出版信息

Colloids Surf B Biointerfaces. 2005 Feb 10;40(2):75-81. doi: 10.1016/j.colsurfb.2004.05.009.

DOI:10.1016/j.colsurfb.2004.05.009

PMID:15642457

Abstract

The paraoxon hydrolysis reaction catalyzed by organophosphorus hydrolase (OPH) monolayer at the air-water interface was studied. OPH-paraoxon interactions, occurring at the two-dimensional interface, by close-packed, highly orientated OPH monolayer, were investigated by several different surface chemistry techniques; e.g. surface pressure area isotherms, atomic force microscopy (AFM), and in situ epifluorescence microscopy. The characterization of OPH Langmuir and Langmuir-Blodgett films prepared in both the presence and absence of paraoxon, demonstrated significantly distinctive feature when compared with one another. Continuous growth of the OPH aggregates is a distinct phenomenon associated with hydrolysis, in addition to the pH changes in the local environment of the enzyme macromolecules.

摘要

研究了空气-水界面处有机磷水解酶（OPH）单层催化对氧磷的水解反应。通过几种不同的表面化学技术研究了在二维界面处由紧密堆积、高度取向的OPH单层发生的OPH-对氧磷相互作用；例如表面压力-面积等温线、原子力显微镜（AFM）和原位落射荧光显微镜。对在有和没有对氧磷的情况下制备的OPH朗缪尔膜和朗缪尔-布洛杰特膜的表征表明，相互比较时具有明显不同的特征。除了酶大分子局部环境中的pH变化外，OPH聚集体的持续生长是与水解相关的一个独特现象。