Cell culture-based assay of parvovirus B19 and the relevance of animal model viruses.

One of the most challenging problems with the interpretation of virus validation studies is the relevance of the model viruses investigated. There are cases where model viruses have been shown to predict the inactivation/removal of a specific virus while unexpected results were obtained in other cases such as inactivation of parvovirus B19 by pasteurisation or treatment with guanidinium hydrochloride. The understanding of the mechanism underlying virus inactivation/removal might be helpful for the selection of model viruses and for the assessment of validation data on its relevance for a specific virus in question.