人甲状旁腺中的外周型苯二氮䓬受体：在腺瘤中上调。

Peripheral type benzodiazepine receptor in human parathyroid glands: up-regulation in adenoma.

作者信息

Giusti L, Costa B, Viacava P, Castagna M, Iacconi P, Ricci R E, Zaccagnini M, Miccoli P, Lucacchini A

机构信息

Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, University of Pisa, Pisa, Italy.

出版信息

J Endocrinol Invest. 2004 Oct;27(9):826-31. doi: 10.1007/BF03346276.

DOI:10.1007/BF03346276

PMID:15648546

Abstract

In this study we report the presence of peripheral benzodiazepine receptors (PBRs) in human parathyroid glands and describe the effect of their benzodiazepine type ligands on parathyroid cell function. PBR binding features in normal parathyroid tissue were characterized and compared to parathyroid adenoma, using a specific and selective ligand for PBR, [3H] 1-(2-chlorophenyl)-N-methyl-N-(1-methyl-propyl)-3-isoquinoline-carboxamide ([3H]PK11195). Affinity and density of [3H]PK11195 binding sites in homogenate membrane preparations from adenomatous and normal tissues were determined. Parathyroid adenoma showed a statistically significant 2.2 fold increase of [3H]PK11195 binding sites, while the affinity remained unchanged. Our results represent the first evidence of PBRs in parathyroid glands and suggest for them a role in influencing PTH release. A clear trend of PBR up-regulation in parathyroid adenoma was also found.

摘要

在本研究中，我们报告了人甲状旁腺中存在外周苯二氮䓬受体（PBRs），并描述了其苯二氮䓬类配体对甲状旁腺细胞功能的影响。使用PBR的特异性和选择性配体[3H]1-(2-氯苯基)-N-甲基-N-(1-甲基丙基)-3-异喹啉甲酰胺([3H]PK11195)，对正常甲状旁腺组织中的PBR结合特征进行了表征，并与甲状旁腺腺瘤进行了比较。测定了腺瘤组织和正常组织匀浆膜制剂中[3H]PK11195结合位点的亲和力和密度。甲状旁腺腺瘤显示[3H]PK11195结合位点有统计学意义的2.2倍增加，而亲和力保持不变。我们的结果首次证明了甲状旁腺中存在PBRs，并提示它们在影响甲状旁腺激素（PTH）释放中起作用。还发现甲状旁腺腺瘤中PBR上调有明显趋势。