拟南芥中两种茄尼基二磷酸合酶的鉴定及亚细胞定位

Identification and subcellular localization of two solanesyl diphosphate synthases from Arabidopsis thaliana.

作者信息

Jun Luo, Saiki Ryoichi, Tatsumi Kei, Nakagawa Tsuyoshi, Kawamukai Makoto

机构信息

Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, 690-8504 Japan.

出版信息

Plant Cell Physiol. 2004 Dec;45(12):1882-8. doi: 10.1093/pcp/pch211.

DOI:10.1093/pcp/pch211

PMID:15653808

Abstract

Two solanesyl diphosphate synthases, designated SPS1 and SPS2, which are responsible for the synthesis of the isoprenoid side chain of either plastoquinone or ubiquinone in Arabidopsis thaliana, were identified. Heterologous expression of either SPS1 or SPS2 allowed the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli. SPS1-GFP was found to localize in the ER while SPS2-GFP localized in the plastid of tobacco BY-2 cells. These two different subcellular localizations are thought to be the reflection of their roles in solanesyl diphosphate synthesis in two different parts: presumably SPS1 and SPS2 for the side chains of ubiquinone and plastoquinone, respectively.

摘要

在拟南芥中鉴定出了两种八氢番茄红素二磷酸合酶，分别命名为SPS1和SPS2，它们负责合成质体醌或泛醌的类异戊二烯侧链。在裂殖酵母的十聚异戊二烯二磷酸合酶缺陷型菌株以及野生型大肠杆菌中，SPS1或SPS2的异源表达都能产生UQ-9。发现SPS1-GFP定位于内质网，而SPS2-GFP定位于烟草BY-2细胞的质体中。这两种不同的亚细胞定位被认为反映了它们在两个不同部位参与八氢番茄红素二磷酸合成中的作用：推测SPS1和SPS2分别参与泛醌和质体醌侧链的合成。