傅里叶变换拉曼光谱法同时测定蜂蜜中的果糖和葡萄糖。

FT-Raman spectroscopic simultaneous determination of fructose and glucose in honey.

作者信息

Batsoulis Apostolos N, Siatis Nikolaos G, Kimbaris Athanasios C, Alissandrakis Eleftherios K, Pappas Christos S, Tarantilis Petros A, Harizanis Paschalis C, Polissiou Moschos G

机构信息

Laboratory of Chemistry, Department of Science, and Laboratory of Sericulture and Apiculture, Department of Crop Science, Agricultural University of Athens, 75 Iera odos, 118 55 Athens, Greece.

出版信息

J Agric Food Chem. 2005 Jan 26;53(2):207-10. doi: 10.1021/jf048793m.

DOI:10.1021/jf048793m

PMID:15656650

Abstract

A new method for mass percentage determination of fructose and glucose based on FT-Raman spectroscopy is evaluated with a standard HPLC-based method. FT-Raman spectra manipulation is done via the spectrometer software, and a PLS (partial least squares) method is developed with the TQ Analyst software (Ver 1. 1a). The simultaneous quantitative determination uses an input range from 1700 to 700 cm(-1) without correction or baseline factors. The standards used in the PLS method are honey samples previously analyzed by HPLC to obtain their mass percentage concentrations in fructose and glucose. The returned results are statistically tested with those of the HPLC method. Both methods appear to score equally in terms of reproducibility. The honey content of the two sugars in total was found up to 40-74%. The honey samples content in fructose and glucose was determined by HPLC (24.1-42.9% and 16.2-33.1%, respectively) and FT-Raman (24.0-40.8% and 21.1-32.2%, respectively).

摘要

基于傅里叶变换拉曼光谱法测定果糖和葡萄糖质量百分比的新方法，采用基于高效液相色谱的标准方法进行评估。傅里叶变换拉曼光谱的处理通过光谱仪软件完成，并使用TQ Analyst软件（版本1.1a）开发了偏最小二乘法（PLS）。同时定量测定使用1700至700 cm(-1) 的输入范围，无需校正或基线因子。PLS方法中使用的标准品是先前通过高效液相色谱分析的蜂蜜样品，以获得其果糖和葡萄糖的质量百分比浓度。将返回的结果与高效液相色谱法的结果进行统计学检验。两种方法在重现性方面似乎得分相同。发现两种糖的蜂蜜总含量高达40 - 74%。通过高效液相色谱法（分别为24.1 - 42.9%和16.2 - 33.1%）和傅里叶变换拉曼光谱法（分别为24.0 - 40.8%和21.1 - 32.2%）测定蜂蜜样品中果糖和葡萄糖的含量。