Suppr超能文献

骨髓作为循环CXCR4+组织定向干细胞的来源。

Bone marrow as a source of circulating CXCR4+ tissue-committed stem cells.

作者信息

Kucia Magda, Ratajczak Janina, Ratajczak Mariusz Z

机构信息

Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, KY 40202, USA.

出版信息

Biol Cell. 2005 Feb;97(2):133-46. doi: 10.1042/BC20040069.

Abstract

Several studies have suggested that adult haematopoietic stem cells (HSCs) may be capable of transdifferentiating across tissue-lineage boundaries, giving rise to the concept that these stem cells are plastic in their differentiative capacity. This topic created much excitement in the scientific community, with the prospect of employing HSCs in tissue/organ regeneration (e.g. heart infarct, stroke, liver damage) as an alternative to multipotent embryonic stem cells. However, recent observations, and several alternative explanations of previously published data (e.g. cell fusion, epigenetic changes), do not support the concept of HSC plasticity. Our recent studies, in which we employed chemotactic isolation to a stromal-cell-derived-factor-1 (SDF-1) gradient combined with real-time reverse transcriptase (RT)-PCR/immuno-histochemical analyses, revealed that bone marrow (BM) contains a highly mobile population of CXCR4+ cells that express mRNA/proteins for various markers of early tissue-committed stem cells (TCSCs). Based on this we postulate that the BM is not only a home for HSCs, but also a 'hideout' for non-haematopoietic CXCR4+ TCSCs, and we suggest that their presence in BM tissue should be considered before experimental evidence is interpreted simply as transdifferentiation/plasticity of HSCs. Furthermore, our observation that the number of TCSCs is the highest in BM of young animals and decreases with age provides a novel insight into aging, and may explain why the regeneration process becomes less effective in older individuals.

摘要

多项研究表明,成体造血干细胞(HSC)可能能够跨组织谱系边界进行转分化,从而产生了这些干细胞在分化能力上具有可塑性的概念。这一话题在科学界引发了极大的兴奋,因为有望利用HSC进行组织/器官再生(如心肌梗死、中风、肝损伤),以替代多能胚胎干细胞。然而,最近的观察结果以及对先前发表数据的几种其他解释(如细胞融合、表观遗传变化)并不支持HSC可塑性的概念。我们最近的研究采用趋化分离法,使其沿基质细胞衍生因子-1(SDF-1)梯度移动,并结合实时逆转录酶(RT)-PCR/免疫组织化学分析,结果显示骨髓(BM)中含有一群高度可移动的CXCR4+细胞,这些细胞表达早期组织定向干细胞(TCSC)各种标志物的mRNA/蛋白质。基于此,我们推测BM不仅是HSC的家园,也是非造血CXCR4+ TCSC的“藏身之处”,并且我们建议在将实验证据简单解释为HSC的转分化/可塑性之前,应考虑它们在BM组织中的存在。此外,我们观察到TCSC的数量在幼龄动物的BM中最高,并随年龄增长而减少,这为衰老提供了新的见解,并且可能解释了为什么再生过程在老年个体中变得不那么有效。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验