Amanchy Ramars, Kalume Dario Eluan, Pandey Akhilesh
McKusick-Nathans Institute of Genetic Medicine and Department of Biological Chemistry and Oncology, Johns Hopkins University, Baltimore, MD 21218, USA.
Sci STKE. 2005 Jan 18;2005(267):pl2. doi: 10.1126/stke.2672005pl2.
Stable isotope labeling with amino acids in cell culture (SILAC) is a simple and straightforward approach for in vivo incorporation of a tag into proteins for relative quantitation by mass spectrometry. SILAC is a simple, yet powerful, method for investigating the dynamics of protein abundance and posttranslational modifications. Here, we provide detailed instructions for using this method to study protein complexes, protein-protein interactions, and the dynamics of protein abundance and posttranslational modifications. We expect that SILAC will become a routine technique because of its applicability to most areas of cell biology. We have also developed a Web site (http://www.silac.org) to provide researchers with updated information about this method and related resources.
细胞培养中氨基酸的稳定同位素标记（SILAC）是一种简单直接的方法，用于在体内将标签掺入蛋白质中，以便通过质谱进行相对定量。SILAC是一种简单但强大的方法，用于研究蛋白质丰度和翻译后修饰的动态变化。在这里，我们提供使用该方法研究蛋白质复合物、蛋白质-蛋白质相互作用以及蛋白质丰度和翻译后修饰动态变化的详细说明。我们预计SILAC将因其适用于细胞生物学的大多数领域而成为一种常规技术。我们还开发了一个网站（http://www.silac.org），为研究人员提供有关该方法和相关资源的最新信息。
