哺乳动物减数分裂中未配对染色质的沉默及组蛋白H2A泛素化
Silencing of unpaired chromatin and histone H2A ubiquitination in mammalian meiosis.
作者信息
Baarends Willy M, Wassenaar Evelyne, van der Laan Roald, Hoogerbrugge Jos, Sleddens-Linkels Esther, Hoeijmakers Jan H J, de Boer Peter, Grootegoed J Anton
机构信息
Department of Reproduction and Development, Erasmus MC, University Medical Center Rotterdam, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands.
出版信息
Mol Cell Biol. 2005 Feb;25(3):1041-53. doi: 10.1128/MCB.25.3.1041-1053.2005.
During meiotic prophase in male mammals, the X and Y chromosomes are incorporated in the XY body. This heterochromatic body is transcriptionally silenced and marked by increased ubiquitination of histone H2A. This led us to investigate the relationship between histone H2A ubiquitination and chromatin silencing in more detail. First, we found that ubiquitinated H2A also marks the silenced X chromosome of the Barr body in female somatic cells. Next, we studied a possible relationship between H2A ubiquitination, chromatin silencing, and unpaired chromatin in meiotic prophase. The mouse models used carry an unpaired autosomal region in male meiosis or unpaired X and Y chromosomes in female meiosis. We show that ubiquitinated histone H2A is associated with transcriptional silencing of large chromatin regions. This silencing in mammalian meiotic prophase cells concerns unpaired chromatin regions and resembles a phenomenon described for the fungus Neurospora crassa and named meiotic silencing by unpaired DNA.
在雄性哺乳动物减数分裂前期，X和Y染色体被纳入XY体。这个异染色质体在转录上是沉默的，并且以组蛋白H2A泛素化增加为特征。这促使我们更详细地研究组蛋白H2A泛素化与染色质沉默之间的关系。首先，我们发现泛素化的H2A也标记了雌性体细胞巴氏小体中沉默的X染色体。接下来，我们研究了减数分裂前期H2A泛素化、染色质沉默和未配对染色质之间的可能关系。所使用的小鼠模型在雄性减数分裂中携带一个未配对的常染色体区域，或在雌性减数分裂中携带未配对的X和Y染色体。我们表明，泛素化的组蛋白H2A与大染色质区域的转录沉默相关。哺乳动物减数分裂前期细胞中的这种沉默涉及未配对的染色质区域，类似于在真菌粗糙脉孢菌中描述的一种现象，称为未配对DNA介导的减数分裂沉默。
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