Morikawa Masa-aki, Yoshihara Masakuni, Endo Takeshi, Kimizuka Nobuo
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Chemistry. 2005 Feb 18;11(5):1574-8. doi: 10.1002/chem.200400751.
alpha-Helical peptide microcapsules were prepared by the emulsion-templated self-assembly of amphiphilic poly(gamma-benzyl L-glutamate)s (PBLG) 1. By mixing solutions of 1 in dichloromethane (in the form of a sodium salt) with water, oil-in-water emulsions were obtained. Spontaneous stripping of the dichloromethane phase caused a decrease in the diameter of the microdroplets and finally stable microcapsules formed. The microcapsules contain an inner aqueous phase as observed by confocal laser scanning microscopy (CLSM). Binding of hydrophobic pyrene molecules to the polypeptide shell was also demonstrated. The present polypeptide microcapsules are stable even after drying in air and they would serve as supramolecular vehicles for both hydrophobic and water-soluble molecules.
通过两亲性聚（γ-苄基-L-谷氨酸）（PBLG）1的乳液模板自组装制备了α-螺旋肽微胶囊。将1在二氯甲烷（钠盐形式）中的溶液与水混合，得到水包油乳液。二氯甲烷相的自发剥离导致微滴直径减小，最终形成稳定的微胶囊。通过共聚焦激光扫描显微镜（CLSM）观察到微胶囊含有内部水相。还证明了疏水性芘分子与多肽壳的结合。目前的多肽微胶囊即使在空气中干燥后也很稳定，它们将作为疏水性和水溶性分子的超分子载体。
