一种来自嗜热芽孢杆菌属的耐热性葡糖淀粉酶：特性与热稳定性

A thermostable glucoamylase from a thermophilic Bacillus sp.: characterization and thermostability.

作者信息

Gill Rupinder K, Kaur Jagdeep

机构信息

Department of Biotechnology, Panjab University, Chandigarh, 160 014, India.

出版信息

J Ind Microbiol Biotechnol. 2004 Dec;31(11):540-3. doi: 10.1007/s10295-004-0185-y. Epub 2004 Nov 18.

DOI:10.1007/s10295-004-0185-y

PMID:15672284

Abstract

A thermostable glucoamylase (GA) showed optimum activity at 70 degrees C and pH 5.0. It was highly stable at pH 7.0. The half-life of the enzyme at pH 7.0 was 13, 8, and 3 h 40 min at 60, 65, and 70 degrees C respectively. The residual activity of the enzyme sample incubated at 5 psi (110 degrees C) for 30 min was about 32% of the control set (incubated at 4 degrees C), while no activity was observed at 10 and 15 psi. The thermostability of the enzyme was enhanced twofold in the presence of 0.5% (w/v) starch at 5 psi. Thin-layer chromatography indicated that this enzyme is a GA.

摘要

一种热稳定的葡糖淀粉酶（GA）在70℃和pH 5.0时表现出最佳活性。它在pH 7.0时高度稳定。该酶在pH 7.0时的半衰期在60℃、65℃和70℃分别为13小时、8小时和3小时40分钟。在5磅力/平方英寸（110℃）下孵育30分钟的酶样品的残余活性约为对照组（在4℃下孵育）的32%，而在10和15磅力/平方英寸下未观察到活性。在5磅力/平方英寸下，0.5%（w/v）淀粉存在时，该酶的热稳定性提高了两倍。薄层色谱表明这种酶是一种葡糖淀粉酶。