Hill J E, Kelley L C, Langheinrich K A
Clemson University Animal Diagnostic Laboratory, Columbia, South Carolina 29224.
Avian Dis. 1992 Jan-Mar;36(1):172-6.
Visceral granulomas containing gram-positive filamentous bacteria were diagnosed in eight chickens collected from processing plants over a period of 4 years. Formalin-fixed tissues were submitted from the chickens after slaughter, but no specimens suitable for bacterial culture were received. The bacteria lacked similar tinctorial, morphologic, and ultrastructural features of other gram-positive bacteria that have been identified in chickens. The bacteria were visible with hematoxylin and eosin and faintly visible with Gomori methenamine-silver, but negative with periodic acid-Schiff and Fite-Faraco acid-fast stains. Granulomas were found in the liver, spleen, and peritoneum.
在4年时间里,从加工厂收集的8只鸡被诊断出患有含有革兰氏阳性丝状细菌的内脏肉芽肿。这些鸡宰杀后提交了福尔马林固定组织,但未收到适合细菌培养的标本。这些细菌缺乏已在鸡中鉴定出的其他革兰氏阳性细菌的类似染色、形态和超微结构特征。苏木精-伊红染色可见这些细菌,Gomori六胺银染色隐约可见,但过碘酸-希夫染色和Fite-Faraco抗酸染色均为阴性。肉芽肿见于肝脏、脾脏和腹膜。