黑绿豆（Vigna mungo L. Hepper）发育种子中凝集素的体内合成与积累。

The in vivo synthesis and accumulation of lectin in developing seeds of black gram (Vigna mungo L. Hepper).

作者信息

Suseelan K N, Mitra R, Bhatia C R, Gopalakrishna T

机构信息

Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay. Mumbai-400 085, India.

出版信息

Plant Foods Hum Nutr. 2004 Summer;59(3):123-8. doi: 10.1007/s11130-004-0050-1.

DOI:10.1007/s11130-004-0050-1

PMID:15678718

Abstract

Black gram (Vigna mungo L. Hepper) seed contains two D-galactose-specific lectin species, BGL-I and BGL-II, identified on the basis of elution from ion exchange column and immunochemical cross-reactivity. BGL-I consisted of two monomeric lectins, BGL-I-1 and BGL-1-2, of relative molecular weights 94 and 89 kDa, respectively. BGL-II is another monomeric lectin with a molecular weight of 83 kDa. The in vivo synthesis studies using pulse-chase experiment showed that BGL-II lectin was synthesized as early as 14 days after flowering (DAF). The 94-kDa BGL-I-1 lectin was synthesized around 17 DAF. There was no cotranslational or posttranslational modification of the lectin proteins. The amount of lectin in developing seeds was determined by radial immunodiffusion assay technique. The maximum amount of lectin per seed was found at 28 DAF.

摘要

黑绿豆（Vigna mungo L. Hepper）种子含有两种D-半乳糖特异性凝集素，即BGL-I和BGL-II，这是根据它们在离子交换柱上的洗脱情况和免疫化学交叉反应性鉴定出来的。BGL-I由两种单体凝集素组成，分别是相对分子质量为94 kDa的BGL-I-1和89 kDa的BGL-1-2。BGL-II是另一种分子量为83 kDa的单体凝集素。使用脉冲追踪实验进行的体内合成研究表明，BGL-II凝集素早在开花后14天（DAF）就开始合成。94 kDa的BGL-I-1凝集素在大约17 DAF时合成。凝集素蛋白没有共翻译或翻译后修饰。通过径向免疫扩散测定技术测定发育种子中凝集素的含量。发现每粒种子凝集素的最大含量出现在28 DAF时。