人凝集素样氧化型低密度脂蛋白受体-1在毕赤酵母中的克隆与表达

Cloning and expression of human lectin-like oxidized low density lipoprotein receptor-1 in Pichia pastoris.

作者信息

Huang Zhentai, Zhang Tiantai, Yang Jinling, Zhu Ping, Du Guanhua, Cheng Kedi

机构信息

Department of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences & Peking Union Medical College, 100050, Beijing, P.R. China.

出版信息

Biotechnol Lett. 2005 Jan;27(1):49-52. doi: 10.1007/s10529-004-6582-5.

DOI:10.1007/s10529-004-6582-5

PMID:15685419

Abstract

Lectin-like oxidatively-modified LDL receptor-1 (LOX-1) is a major receptor for oxidized low-density lipoprotein (oxLDL) in aortic endothelial cells. Human LOX-1 (hLOX-1) gene (cDNA) was cloned from the monocytic leukemic cell line THP-1 and expressed in Pichia pastoris. The recombinant protein (rhLOX-1) was purified by his-tag affinity chromatography. Preliminary identification was performed by Western blot analysis and a ligand-receptor binding assay showed that the protein had specific oxLDL-binding activity.

摘要

凝集素样氧化修饰低密度脂蛋白受体-1（LOX-1）是主动脉内皮细胞中氧化型低密度脂蛋白（oxLDL）的主要受体。人LOX-1（hLOX-1）基因（cDNA）从单核细胞白血病细胞系THP-1中克隆出来，并在毕赤酵母中表达。重组蛋白（rhLOX-1）通过组氨酸标签亲和层析进行纯化。通过蛋白质免疫印迹分析进行初步鉴定，配体-受体结合试验表明该蛋白具有特异性oxLDL结合活性。