George Lisle W, Borrowman Andrew J, Angelos John A
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Am J Vet Res. 2005 Jan;66(1):136-42. doi: 10.2460/ajvr.2005.66.136.
To determine the immunogenicity of a Moraxella bovis cytolysin-enriched vaccine for prevention of infectious bovine keratoconjunctivitis (IBK).
104 mixed-breed beef calves ranging between 4 and 8 months of age.
Vaccines were prepared by the diafiltration of broth culture supernatant from hemolytic M bovis or sterile media. The diafiltered retentate was combined with Quil A adjuvant. Calves were randomly assigned to receive either the cytolysin vaccine (n = 35) or, as controls, adjuvant (35) or saline (0.9% NaCl) solution (34). Eyes of all calves were examined weekly for signs of IBK for 15 weeks. Calves that developed severe IBK were treated SC with florfenicol.
Cytolysin vaccine contained 4 proteins with molecular masses ranging between 65 and 90 kd. Cytolysin-vaccinated calves had fewer instances of IBK than control calves. The time of onset of corneal lesions in cytolysin-vaccinated calves that developed IBK was delayed, compared with that of calves in either control group. The cytolysin-Quil A vaccine contained endotoxin, but calves did not have clinical signs of illness after vaccination.
Calves that were vaccinated with a cytolysin-enriched vaccine had some resistance to IBK. Vaccines containing concentrated diafiltered M bovis cytolysin could protect beef calves against IBK.
确定一种富含牛莫拉菌溶细胞素的疫苗预防牛传染性角膜结膜炎（IBK）的免疫原性。
104头4至8月龄的杂种肉牛犊。
通过对溶血性牛莫拉菌肉汤培养上清液或无菌培养基进行渗滤来制备疫苗。渗滤后的截留物与Quil A佐剂混合。犊牛被随机分配接受溶细胞素疫苗（n = 35），或作为对照，接受佐剂（35头）或生理盐水（0.9% NaCl）溶液（34头）。每周检查所有犊牛的眼睛，持续15周，观察是否有IBK迹象。发生严重IBK的犊牛用氟苯尼考进行皮下治疗。
溶细胞素疫苗含有4种分子量在65至90 kd之间的蛋白质。接种溶细胞素疫苗的犊牛患IBK的情况比对照犊牛少。与任何一个对照组的犊牛相比，接种溶细胞素疫苗且发生IBK的犊眼角膜病变的发病时间延迟。溶细胞素 - Quil A疫苗含有内毒素，但犊牛接种后没有疾病的临床症状。
接种富含溶细胞素疫苗的犊牛对IBK有一定抵抗力。含有浓缩渗滤牛莫拉菌溶细胞素的疫苗可以保护肉牛犊免受IBK感染。
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