兔眼准分子原位角膜磨镶术和准分子激光角膜切削术后角膜对噻吗洛尔通透性的变化

Change in corneal permeability to timolol after laser in situ keratomileusis and photorefractive keratectomy in rabbit.

作者信息

Hamada Naoki, Amano Shiro, Yamagami Satoru, Araie Makoto, Oshika Tetsuro, Wada Takahiro, Miyai Hiroko, Notsu Yoshitada

机构信息

Department of Ophthalmology, University of Tokyo School of Medicine, Tokyo 113-8655, Japan.

出版信息

Jpn J Ophthalmol. 2005 Jan-Feb;49(1):12-4. doi: 10.1007/s10384-004-0142-3.

DOI:10.1007/s10384-004-0142-3

PMID:15692768

Abstract

PURPOSE

To evaluate the change in corneal permeability to timolol 1 month after laser in situ keratomileusis (LASIK) or photorefractive keratectomy (PRK) in rabbits.

METHODS

LASIK or PRK was performed on one eye of each of 20 rabbits. One month later, 20 microl of 0.5% timolol was instilled into the 10 LASIK eyes, 10 PRK eyes, and the 20 control contralateral eyes. One hour later, 150 microl of aqueous humor was collected from all eyes, and the concentration of timolol in the aqueous humor was measured by high-performance liquid chromatography. The effect of LASIK and PRK on the corneal permeability to timolol was analyzed.

RESULTS

The average timolol concentrations in the aqueous humor of the ten eyes 1 month after LASIK and in the ten contralateral eyes were 1.16 +/- 0.58 microg/ml and 1.07 +/- 0.39 microg/ml (average +/- SD, P = 0.678), respectively. The average timolol concentrations in the aqueous humor of the ten eyes 1 month after PRK and in the ten contralateral eyes were 1.24 +/- 0.43 microg/ml and 1.12 +/- 0.30 microg/ml (P = 0.483), respectively. The average ratio of timolol concentration in the aqueous humor of the LASIK or PRK eyes 1 month after surgery to that of the contralateral normal eyes was 1.18 +/- 0.58 or 1.19 +/- 0.67 (P = 0.972), respectively.

CONCLUSIONS

LASIK and PRK do not affect corneal permeability to timolol 1 month after surgery.

摘要

目的

评估兔眼准分子原位角膜磨镶术（LASIK）或准分子激光角膜切削术（PRK）1个月后角膜对噻吗洛尔通透性的变化。

方法

对20只兔的每只眼睛中的一只进行LASIK或PRK手术。1个月后，将20微升0.5%噻吗洛尔滴入10只接受LASIK手术的眼睛、10只接受PRK手术的眼睛以及20只对侧对照眼睛。1小时后，从所有眼睛中采集150微升房水，采用高效液相色谱法测量房水中噻吗洛尔的浓度。分析LASIK和PRK对角膜对噻吗洛尔通透性的影响。

结果

LASIK术后1个月，10只眼睛房水中噻吗洛尔的平均浓度和10只对侧眼睛分别为1.16±0.58微克/毫升和1.07±0.39微克/毫升（平均值±标准差，P = 0.678）。PRK术后1个月，10只眼睛房水中噻吗洛尔的平均浓度和10只对侧眼睛分别为1.24±0.43微克/毫升和1.12±0.30微克/毫升（P = 0.483）。LASIK或PRK术后1个月，手术眼房水中噻吗洛尔浓度与对侧正常眼房水中噻吗洛尔浓度的平均比值分别为1.18±0.58或1.19±0.67（P = 0.972）。