Stavisky Ronda C, Britt Joshua M, Zuzek Aleksej, Truong Elizabeth, Bittner George D
Section of Neurobiology, The University of Texas at Austin, 1 University Station 0C0920, Austin, TX, 78712-0248, USA.
Neurosci Lett. 2005 Mar 11;376(2):98-101. doi: 10.1016/j.neulet.2004.11.033. Epub 2004 Dec 15.
This study examines the effects of several experimental compounds [melatonin (MEL), cyclosporin A (CsA), glial-derived neurotrophic factor (GDNF), and methylprednisolone (MP)] on polyethylene glycol (PEG)-induced repair in vitro and/or in vivo by plasmalemmal fusion (PEG-fusion) of sciatic axons severed by crushing. As measured by conduction of compound action potentials (CAPs) through the lesion site, a significantly (p<0.025) higher percentage (75%) of crushed rat sciatic axons can be repaired in vitro by PEG-fusion following exposure to MEL compared to PEG-fusion of severed sciatic axons in control Krebs saline that contains calcium (CTL=20%). In contrast, no other experimental compound (GDNF: 45%; MP: 42%; CsA: 24%) produces a significant improvement in PEG-fusion success compared to CTL. Further, MEL produces significantly (p<0.001) larger peak CAP amplitudes conducted through the lesion site following PEG-fusion compared to CTL or any other experimental compound in vitro. Additionally, MEL significantly (p<0.025) increases the ability to PEG-fuse sciatic axons in vivo, compared to CTL. Finally, PEG-fusion success in vivo is significantly (p<0.01) greater in calcium-free CTL (CTL-Ca) compared to CTL.
本研究考察了几种实验性化合物[褪黑素(MEL)、环孢素A(CsA)、胶质细胞源性神经营养因子(GDNF)和甲基强的松龙(MP)]对通过挤压切断的坐骨神经轴突经质膜融合(PEG融合)在体外和/或体内进行聚乙二醇(PEG)诱导修复的影响。通过复合动作电位(CAP)在损伤部位的传导来测量,与在含钙的对照Krebs盐溶液(CTL = 20%)中切断的坐骨神经轴突的PEG融合相比,暴露于MEL后,经PEG融合在体外可修复的挤压大鼠坐骨神经轴突的百分比显著更高(p < 0.025)(75%)。相比之下,与CTL相比,没有其他实验性化合物(GDNF:45%;MP:42%;CsA:24%)在PEG融合成功率方面产生显著改善。此外,与CTL或体外的任何其他实验性化合物相比,MEL在PEG融合后通过损伤部位传导的CAP峰值幅度显著更大(p < 0.001)。另外,与CTL相比,MEL在体内显著提高了PEG融合坐骨神经轴突的能力(p < 0.025)。最后,与CTL相比,无钙CTL(CTL-Ca)在体内的PEG融合成功率显著更高(p < 0.01)。