Liu Tai-Yun, Zhang Cheng, Xiao Lu-Lu, Yao Xiao-Li, Feng Shan-Wei, Zeng Ying
Department of Neurology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510089, China.
Di Yi Jun Yi Da Xue Xue Bao. 2005 Feb;25(2):152-5.
To probe the conditions for inducing human cord blood monocytes to differentiate into neuron-like cells.
The mononuclear cells were isolated from human umbilical cord blood samples and plated in 25-mm culture flasks containing DMEM/F12 medium. The fifth passage of mesenchymal stem cells (MSCs) were induced by beta-mercaptoe- thanol (beta-ME), dimethyl sulfoxide (DMSO) and conditioned medium for neuron induction, respectively, to differentiate into neuron-like cells. The expression of nestin, neuron-specific enolase (NSE) and neurofilament (NF) on the treated cells were detected by immunocytochemical method.
Nestin expression were found in 6.7% of the primary, 12.4% of the second passage, 20.8% of the fifth passage of MSCs, respectively. Thirty-six percent of cell cultures treated with the conditioned medium for neuron induction were immunoreactive for NSE and NF, and 33% of the cells induced by beta-ME and 25% of the cells by DMSO also expressed NSE and NF.
Cord blood MSCs possess some features of neural stem cells, and have the capacity to differentiate into neuron-like cells under proper conditions.
探讨诱导人脐血单核细胞分化为神经元样细胞的条件。
从人脐带血样本中分离单核细胞，接种于含有DMEM/F12培养基的25 mm培养瓶中。分别用β-巯基乙醇（β-ME）、二甲基亚砜（DMSO）和神经元诱导条件培养基诱导第5代间充质干细胞（MSCs）分化为神经元样细胞。采用免疫细胞化学方法检测处理后细胞中巢蛋白、神经元特异性烯醇化酶（NSE）和神经丝（NF）的表达。
巢蛋白表达分别在原代MSCs的6.7%、第2代的12.4%、第5代的20.8%中被发现。用神经元诱导条件培养基处理的细胞培养物中，36%对NSE和NF呈免疫反应性，β-ME诱导的细胞中有33%、DMSO诱导的细胞中有25%也表达NSE和NF。
脐血MSCs具有神经干细胞的一些特性，在适当条件下有分化为神经元样细胞的能力。
