Synthesis of homogenous site-selectively glycosylated proteins.
作者信息
Swanwick Richard S, Daines Alison M, Flitsch Sabine L, Allemann Rudolf K
机构信息
School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
出版信息
Org Biomol Chem. 2005 Feb 21;3(4):572-4. doi: 10.1039/b417133k. Epub 2004 Dec 24.
DOI:10.1039/b417133k
PMID:15703788
Abstract
Apparently homogenous glycoproteins can be synthesised in good yield by a combination of site directed mutagenesis, a highly flexible but selective chemical derivatisation and efficient purification through the use of glycosyl thiosulfonates such as 2-((biotinoyl)-amino)-ethyl methanethiosulfonate.
摘要
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