Gao Li-fang, Xu De-qi, Shao Yue-ting, Zhao Dan, Zhao Xue-jian
Department of Pathophysiology, School of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
Zhonghua Nan Ke Xue. 2005 Jan;11(1):29-33, 37.
To study the effects of pSilencer 1.0-U6-siRNA-STAT3 on the growth of PC3 and LNCaP cells.
Three pairs of DNA template coding siRNA were synthesized against STAT3 to reconstruct pSilencer 1.0-U6-STAT3-siRNA, which was transfected into PC3 and LNCaP cells. The STAT3 expression in PC3 cells and LNCaP were transfected with pSilencer 1.0-U6-siRNA-STAT3, and it was detected by Western blot and Northern blot. MTT and FCM were used to observe the growth-inhibiting ratio and apoptosis in PC3 cells.
Western blot and Northern blot analyses demonstrated that pSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit the expression of STAT3 in PC3 and LNCaP cells; MIT and FCM results showed that it could suppress the growth of PC3 cells and LNCaP and induce apoptosis of PC3 cells in vitro.
PSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit STAT3 expression, suppress the growth of PC3 and LNCaP cells and induce the apoptosis of PC3 cells.
研究pSilencer 1.0-U6-siRNA-STAT3对PC3和LNCaP细胞生长的影响。
合成针对信号转导与转录激活因子3（STAT3）的三对编码小干扰RNA（siRNA）的DNA模板，用于构建pSilencer 1.0-U6-STAT3-siRNA，并将其转染至PC3和LNCaP细胞。用pSilencer 1.0-U6-siRNA-STAT3转染PC3细胞和LNCaP细胞后，通过蛋白质免疫印迹法（Western blot）和Northern印迹法检测STAT3的表达。采用噻唑蓝比色法（MTT）和流式细胞术（FCM）观察PC3细胞的生长抑制率和凋亡情况。
蛋白质免疫印迹法和Northern印迹法分析表明，pSilencer 1.0-U6-siRNA-STAT3可显著抑制PC3和LNCaP细胞中STAT3的表达；MTT法和FCM结果显示，其可在体外抑制PC3细胞和LNCaP细胞的生长，并诱导PC3细胞凋亡。
pSilencer 1.0-U6-siRNA-STAT3可显著抑制STAT3表达，抑制PC3和LNCaP细胞的生长，并诱导PC3细胞凋亡。
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