[真皮模板对伤口愈合过程中成纤维细胞生物学行为的影响]

[Effects of dermal template on the biological behaviors of fibroblasts during wound healing].

作者信息

Liu Yingkai, Lu Shuliang, Qing Chun, Liao Zhenjiang, Shi Jixiang

机构信息

Department of Burns, Burn Institute of Shanghai, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, P R China.

出版信息

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Jan;19(1):10-4.

PMID:15704833

Abstract

OBJECTIVE

To study the effects of dermal template on the biological behaviors of fibroblasts during wound healing.

METHODS

A total of 120 rats were made full-thickness wound modes on the dorsum and divided into 4 groups, in group 1, the wounds were allowed to heal by contraction(ConT); in group 2, the wounds covered with full-thickness skin grafts (FTSG); in group 3, the wounds were with split thickness skin grafts (STSG); and in group 4, the wounds were covered by dermal regeneration template with overlying thin split-thickness autograft (ADMT). The specimens were obtained at one week, two weeks, four weeks, six weeks, and twelve weeks respectively. The expressions of alpha smooth muscle actin(alpha-SMA, characteristic of MFB), fibronectin (FN), integrin alpha2 beta1 and transforming growth factor beta1 (TGF-beta1) were examined by immunohistochemical analysis.

RESULTS

Positive expression of alpha-SMA, FN, integrin alpha2 beta1 and TGF-beta1 in ADMT groups was significantly lower than that in STSG group and ConT group, but higher than that in FTSG group (P < 0.05).

CONCLUSION

Dermal regeneration template can inhibit the transformation of FB to MFB and restrain the expression of FN, integrin alpha2 beta1, and TGF-beta1 in fibroblasts which might reduce the possibility of hypertrophic scarring, and improve wound healing.

摘要

目的

研究真皮模板对伤口愈合过程中成纤维细胞生物学行为的影响。

方法

将120只大鼠背部制成全层伤口模型，分为4组，第1组伤口通过收缩自行愈合（ConT）；第2组伤口覆盖全厚皮片（FTSG）；第3组伤口覆盖断层皮片（STSG）；第4组伤口覆盖真皮再生模板并在其上覆盖薄断层自体皮片（ADMT）。分别在1周、2周、4周、6周和12周获取标本。通过免疫组织化学分析检测α平滑肌肌动蛋白（α-SMA，肌成纤维细胞特征性标志物）、纤连蛋白（FN）、整合素α2β1和转化生长因子β1（TGF-β1）的表达。