糖脂作为苏云金芽孢杆菌晶体毒素的受体

Glycolipids as receptors for Bacillus thuringiensis crystal toxin.

作者信息

Griffitts Joel S, Haslam Stuart M, Yang Tinglu, Garczynski Stephan F, Mulloy Barbara, Morris Howard, Cremer Paul S, Dell Anne, Adang Michael J, Aroian Raffi V

机构信息

Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093-0349, USA.

出版信息

Science. 2005 Feb 11;307(5711):922-5. doi: 10.1126/science.1104444.

DOI:10.1126/science.1104444

PMID:15705852

Abstract

The development of pest resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxins used in transgenic and organic farming. Here, we demonstrate that (i) the major mechanism for Bt toxin resistance in Caenorhabditis elegans entails a loss of glycolipid carbohydrates; (ii) Bt toxin directly and specifically binds glycolipids; and (iii) this binding is carbohydrate-dependent and relevant for toxin action in vivo. These carbohydrates contain the arthroseries core conserved in insects and nematodes but lacking in vertebrates. We present evidence that insect glycolipids are also receptors for Bt toxin.

摘要

害虫抗性的发展威胁到转基因和有机农业中使用的苏云金芽孢杆菌（Bt）毒素的有效性。在此，我们证明：（i）秀丽隐杆线虫中Bt毒素抗性的主要机制是糖脂碳水化合物的缺失；（ii）Bt毒素直接且特异性地结合糖脂；（iii）这种结合依赖于碳水化合物，并且与毒素在体内的作用相关。这些碳水化合物含有昆虫和线虫中保守的节肢动物系列核心，但脊椎动物中缺乏。我们提供证据表明昆虫糖脂也是Bt毒素的受体。