甲基化作为植物微小RNA生物合成中的关键步骤。
Methylation as a crucial step in plant microRNA biogenesis.
作者信息
Yu Bin, Yang Zhiyong, Li Junjie, Minakhina Svetlana, Yang Maocheng, Padgett Richard W, Steward Ruth, Chen Xuemei
机构信息
Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA.
出版信息
Science. 2005 Feb 11;307(5711):932-5. doi: 10.1126/science.1107130.
Abstract
Methylation on the base or the ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and is thought to be crucial for ribosome biogenesis and function. Artificially introduced 2'-O-methyl groups in small interfering RNAs (siRNAs) can stabilize siRNAs in serum without affecting their activities in RNA interference in mammalian cells. Here, we show that plant microRNAs (miRNAs) have a naturally occurring methyl group on the ribose of the last nucleotide. Whereas methylation of rRNAs depends on guide RNAs, the methyltransferase protein HEN1 is sufficient to methylate miRNA/miRNA* duplexes. Our studies uncover a new and crucial step in plant miRNA biogenesis and have profound implications in the function of miRNAs.
摘要
碱基或核糖上的甲基化在真核生物核糖体RNA（rRNA）中普遍存在，并且被认为对核糖体的生物合成和功能至关重要。在小干扰RNA（siRNA）中人工引入的2'-O-甲基基团可以在血清中稳定siRNA，而不影响其在哺乳动物细胞RNA干扰中的活性。在此，我们表明植物微小RNA（miRNA）在最后一个核苷酸的核糖上有一个天然存在的甲基基团。虽然rRNA的甲基化依赖于引导RNA，但甲基转移酶蛋白HEN1足以使miRNA/miRNA*双链体甲基化。我们的研究揭示了植物miRNA生物合成中的一个新的关键步骤，并对miRNA的功能具有深远影响。
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